Protective effect of esculentoside A on radiation-induced dermatitis and fibrosis

Journal Article · Sat Jul 01 00:00:00 EDT 2006 · International Journal of Radiation Oncology, Biology and Physics

DOI:https://doi.org/10.1016/j.ijrobp.2006.01.031· OSTI ID:20842923

Zhenyu, Xiao ^[1]; Ying, Su ^[1]; Shanmin, Yang ^[1]; Liangjie, Yin ^[1]; Wei, Wang ^[1]; Yanghua, Yi ^[1]; Fenton, Bruce M ^[1]; Lurong, Zhang ^[1]; Okunieff, Paul ^[1]

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1. Department of Radiation Oncology, James P. Wilmot Cancer Center at the University of Rochester Medical Center, Rochester, NY (United States)

Purpose: To investigate the effect of esculentoside A (EsA) on radiation-induced cutaneous and fibrovascular toxicity and its possible molecular mechanisms, both in vivo and in vitro. Methods and Materials: Mice received drug intervention 18 hours before 30 Gy to the right hind leg. Alterations in several cytokines expressed in skin tissue 2 days after irradiation were determined by ELISA. Early skin toxicity was evaluated 3 to 4 weeks after irradiation by skin scoring, and both tissue contraction and expression of TGF-{beta}1 were determined for soft-tissue fibrosis 3 months after irradiation. In vitro, the effect of EsA on radiation-induced nitric oxide (NO) and cytokine production in different cell types was measured by application of 2, 4, and 8 Gy. Results: In vivo, EsA reduced levels of IL-1{alpha}, MCP-1, VEGF, and TGF-{beta}1 in cutaneous tissue and reduced soft-tissue toxicity. In vitro, EsA inhibited the IL-1{alpha} ordinarily produced after 4 Gy in A431 cells. In Raw264.7 cells, EsA reduced levels of IL-1{alpha}, IL-1{beta}, and NO production costimulated by radiation and lipopolysaccharide (LPS). In L-929 cells, EsA inhibited VEGF, TNF, and MCP-1 production at 2, 4, and 8 Gy. Conclusions: Esculentoside A protects soft tissues against radiation toxicity through inhibiting the production of several proinflammatory cytokines and inflammatory mediators in epithelial cells, macrophages, fibroblasts, and skin tissue.

• OSTI ID: 20842923
• Journal Information: International Journal of Radiation Oncology, Biology and Physics, Journal Name: International Journal of Radiation Oncology, Biology and Physics Journal Issue: 3 Vol. 65; ISSN IOBPD3; ISSN 0360-3016
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
DERMATITIS
DRUGS
ENZYME IMMUNOASSAY
FIBROBLASTS
FIBROSIS
IN VITRO
IN VIVO
INFLAMMATION
IRRADIATION
LYMPHOKINES
MACROPHAGES
MICE
NITRIC OXIDE
SKIN
TOXICITY