Effect of sulfur dioxide inhalation on CYP2B1/2 and CYP2E1 in rat liver and lung

Journal Article · Sat Jul 15 00:00:00 EDT 2006 · Inhalation Toxicology

OSTI ID:20813173

Qin, Guohua; Meng, Ziqiang ^[1]

---

1. Shanxi University, Taiyuan (China). Institute of Environmental Medicine and Toxicology

Sulfur dioxide (SO{sub 2}) is a ubiquitous air pollutant, present in low concentrations in the urban air and in higher concentrations in the working environment. In this study, we investigated the effects of inhaled SO{sub 2} on the O-dealkylase of pentoxyresorufin (PROD) and p-nitrophenol hydroxylases (p-NP) activities and mRNA levels of CYP2B1/2 and CYP2E1 in the lung and liver of Wistar rats. Male Wistar rats were housed in exposure chambers and treated with 14.11 {+-}1.53, 28.36 {+-} 2.12, and 56.25 {+-} 4.28 mg /m{sup 3}SO{sub 2} for 6 h/day for 7 days, while control rats were exposed to filtered air in the same condition. The mRNAs of CYP2B1/2 and -2E1 were analyzed in livers and lungs by using reverse-transcription polymerase chain reaction (RT-PCR). Results showed that the PROD activities and mRNA of CYP2B1/2 were decreased in livers and lungs of rats exposed to SO{sub 2}. The p-NP activities and mRNA of CYP2E1 were decreased in lungs but not in livers of rats exposed to SO{sub 2}. Total liver microsomal cytochrome P-450 (CYP) contents were diminished in SO{sub 2} -exposed rats. These results lead to two conclusions: (1) SO{sub 2} exposure can suppress CYP2B1/2 and CYP2E1 in lungs and CYP2B1/2 in livers of rats, thus modifying the liver and lung toxication/detoxication potential, and (2) the total liver microsomal CYP contents were diminished, although the activity and mRNA expression of CYP2E1 in rat livers were not affected by SO{sub 2} exposure.

• OSTI ID: 20813173
• Journal Information: Inhalation Toxicology, Vol. 18, Issue 8; ISSN 0895-8378
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
SULFUR DIOXIDE
RATS
ENVIRONMENTAL EXPOSURE
LIVER
INHALATION
LUNGS
CONCENTRATION RATIO
INHIBITION
BIOLOGICAL EFFECTS
NUCLEIC ACIDS
MICROSOMES
ENZYMES
TOXICITY
HEALTH HAZARDS
POLYMERASE CHAIN REACTION
METABOLIC DISEASES
CYTOCHROMES