Protection against avian influenza H9N2 virus challenge by immunization with hemagglutinin- or neuraminidase-expressing DNA in BALB/c mice

Journal Article · Fri May 19 04:00:00 EDT 2006 · Biochemical and Biophysical Research Communications

OSTI ID:20798954

Meizhen, Qiu ^[1]; Fang, Fang ^[1]; Yan, Chen ^[1]; Hualin, Wang ^[2]; Quanjiao, Chen ^[2]; Haiyan, Chang ^[1]; Fuyan, Wang ^[1]; Hanzhong, Wang ^[2]; Ran, Zhang ^[1]; Ze, Chen ^[3]

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1. College of Life Science, Hunan Normal University, Changsha 410081, Hunan (China)
2. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, Hubei (China)
3. College of Life Science, Hunan Normal University, Changsha 410081, Hunan (China) and State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, Hubei (China) and Shanghai Institute of Biological Products, Shanghai 200052 (China)

Avian influenza viruses of H9N2 subtype are widely spread in avian species. The viruses have recently been transmitted to mammalian species, including humans, accelerating the efforts to devise protective strategies against them. In this study, an avian influenza H9N2 virus strain (A/Chicken/Jiangsu/7/2002), isolated in Jiangsu Province, China, was used to infect BALB/c mice for adaptation. After five lung-to-lung passages, the virus was stably proliferated in a large quantity in the murine lung and caused the deaths of mice. In addition, we explored the protection induced by H9N2 virus hemagglutinin (HA)- and neuraminidase (NA)-expressing DNAs in BALB/c mice. Female BALB/c mice aged 6-8 weeks were immunized once or twice at a 3-week interval with HA-DNA and NA-DNA by electroporation, respectively, each at a dose of 3, 10 or 30 {mu}g. The mice were challenged with a lethal dose (40x LD{sub 5}) of influenza H9N2 virus four weeks after immunization once or one week after immunization twice. The protections of DNA vaccines were evaluated by the serum antibody titers, residual lung virus titers, and survival rates of the mice. The result showed that immunization once with not less than 10 {mu}g or twice with 3 {mu}g HA-DNA or NA-DNA provided effective protection against homologous avian influenza H9N2 virus.

• OSTI ID: 20798954
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 343; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANTIBODIES
CHICKENS
DNA
INFLUENZA
INFLUENZA VIRUSES
LETHAL DOSES
LUNGS
MICE
VACCINES