Interleukin-6 triggers human cerebral endothelial cells proliferation and migration: The role for KDR and MMP-9

Journal Article · Fri Apr 21 00:00:00 EDT 2006 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.02.100· OSTI ID:20798913

Yao, Jianhua S ^[1]; Wenwu, Zhai ^[2]; Young, William L ^[1]; Guoyuan, Yang ^[3]

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1. Center for Cerebrovascular Research, Department of Anesthesia and Perioperative Care, University of California, San Francisco, CA 94110 (United States)
2. Department of Lung Biology, University of California, San Francisco, CA 94110 (United States)
3. Center for Cerebrovascular Research, Department of Anesthesia and Perioperative Care, University of California, San Francisco, CA 94110 (United States) and Department of Neurological Surgery, University of California, San Francisco, CA 94110 (United States)

Interleukin-6 (IL-6) is involved in angiogenesis. However, the underlying mechanisms are unknown. Using human cerebral endothelial cell (HCEC), we report for First time that IL-6 triggers HCEC proliferation and migration in a dose-dependent manner, specifically associated with enhancement of VEGF expression, up-regulated and phosphorylated VEGF receptor-2 (KDR), and stimulated MMP-9 secretion. We investigated the signal pathway of IL-6/IL-6R responsible for KDR's regulation. Pharmacological inhibitor of PI3K failed to inhibit IL-6-mediated VEGF overexpression, while blocking ERK1/2 with PD98059 could abolish IL-6-induced KDR overexpression. Further, neutralizing endogenous VEGF attenuated KDR expression and phosphorylation, suggesting that IL-6-induced KDR activation is independent of VEGF stimulation. MMP-9 inhibitor GM6001 significantly decreases HCEC proliferation and migration (p < 0.05), indicating the crucial function of MMP-9 in promoting angiogenic changes in HCECs. We conclude that IL-6 triggers VEGF-induced angiogenic activity through increasing VEGF release, up-regulates KDR expression and phosphorylation through activating ERK1/2 signaling, and stimulates MMP-9 overexpression.

• OSTI ID: 20798913
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 342, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2006.02.100; PII: S0006-291X(06)00409-8; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL PROLIFERATION
GENE REGULATION
GROWTH FACTORS
PHOSPHORYLATION
RECEPTORS
SECRETION
STIMULATION