Macrophage migration inhibitory factor interacts with HBx and inhibits its apoptotic activity

Journal Article · Fri Apr 07 04:00:00 EDT 2006 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.01.180· OSTI ID:20798885

Shimeng, Zhang ^[1]; Ruxian, Lin ^[1]; Zhe, Zhou ^[1]; Siyuan, Wen ^[1]; Li, Lin ^[1]; Suhong, Chen ^[1]; Yajun, Shan ^[1]; Yuwen, Cong ^[1]; Shengqi, Wang ^[1]

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1. Beijing Institute of Radiation Medicine, No.27 Taiping Road, Beijing 100850 (China)

HBx, a transcriptional transactivating protein of hepatitis B virus (HBV), is required for viral infection and has been implicated in virus-mediated liver oncogenesis. However, the precise molecular mechanism remains largely elusive. We used the yeast two-hybrid system to identify that HBx interacts with MIF directly. Macrophage migration inhibitory factor (MIF) is implicated in the regulation of inflammation, cell growth, and even tumor formation. The interaction between HBx and MIF was verified with co-immunoprecipitation, GST pull-down, and cellular colocalization. The expression of MIF was up-regulated in HBV particle producing cell 2.2.15 compared with HepG2 cell. Both HBx and MIF cause HepG2 cell G /G{sub 1} phase arrest, proliferation inhibition, and apoptosis. However, MIF can counteract the apoptotic effect of HBx. These results may provide evidence to explain the link between HBV infection and hepatocellular carcinoma.

• OSTI ID: 20798885
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 342; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
APOPTOSIS
CELL PROLIFERATION
GENE REGULATION
GROWTH
HEPATITIS
HEPATOMAS
INFLAMMATION
INHIBITION
LIVER
MACROPHAGES
PROTEINS
VIRUSES
YEASTS