Wnt-10b promotes differentiation of skin epithelial cells in vitro

Journal Article · Fri Mar 31 04:00:00 EST 2006 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.01.104· OSTI ID:20798863

Ouji, Yukiteru ^[1]; Yoshikawa, Masahide ^[1]; Shiroi, Akira ^[1]; Ishizaka, Shigeaki ^[1]

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1. Program in Tissue Engineering and Department of Parasitology, Nara Medical University, Kashihara, Nara (Japan)

To evaluate the role of Wnt-10b in epithelial differentiation, we investigated the effects of Wnt-10b on adult mouse-derived primary skin epithelial cells (MPSEC). Recombinant Wnt-10b protein (rWnt-10b) was prepared using a gene engineering technique and MPSEC were cultured in its presence, which resulted in morphological changes from cuboidal to spindle-shaped and inhibited their proliferation. Further, involvement of the canonical Wnt signal pathway was also observed. MPSEC treated with rWnt-10b showed characteristics of the hair shaft and inner root sheath of the hair follicle, in results of Ayoub Shklar staining and immunocytochemistry. Further, the cells expressed mRNA for differentiated epithelial cells, including keratin 1, keratin 2, loricrin, mHa5, and mHb5, in association with a decreased expression of the basal cell marker keratin 5. These results suggest that Wnt-10b promotes the differentiation of MPSEC.

• OSTI ID: 20798863
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 342; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ADULTS
CELL PROLIFERATION
GENES
GENETIC ENGINEERING
HAIR
HAIR FOLLICLES
IN VITRO
KERATIN
MICE
MORPHOLOGICAL CHANGES