Human adipose tissue-derived mesenchymal stem cells differentiate into insulin, somatostatin, and glucagon expressing cells

Journal Article · Fri Mar 24 04:00:00 EST 2006 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.01.072· OSTI ID:20798855

Timper, Katharina ^[1]; Seboek, Dalma ^[1]; Eberhardt, Michael ^[1]; Linscheid, Philippe ^[1]; Christ-Crain, Mirjam ^[2]; Keller, Ulrich ^[1]; Mueller, Beat ^[1]; Zulewski, Henryk ^[3]

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1. Department of Research, University Hospital, Basel (Switzerland)
2. Division of Endocrinology, Diabetes and Clinical Nutrition, University Hospital, Basel (Switzerland)
3. Department of Research, University Hospital, Basel (Switzerland) and Division of Endocrinology, Diabetes and Clinical Nutrition, University Hospital, Basel (Switzerland)

Mesenchymal stem cells (MSC) from mouse bone marrow were shown to adopt a pancreatic endocrine phenotype in vitro and to reverse diabetes in an animal model. MSC from human bone marrow and adipose tissue represent very similar cell populations with comparable phenotypes. Adipose tissue is abundant and easily accessible and could thus also harbor cells with the potential to differentiate in insulin producing cells. We isolated human adipose tissue-derived MSC from four healthy donors. During the proliferation period, the cells expressed the stem cell markers nestin, ABCG2, SCF, Thy-1 as well as the pancreatic endocrine transcription factor Isl-1. The cells were induced to differentiate into a pancreatic endocrine phenotype by defined culture conditions within 3 days. Using quantitative PCR a down-regulation of ABCG2 and up-regulation of pancreatic developmental transcription factors Isl-1, Ipf-1, and Ngn3 were observed together with induction of the islet hormones insulin, glucagon, and somatostatin.

• OSTI ID: 20798855
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 341; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ADIPOSE TISSUE
BONE MARROW
CELL PROLIFERATION
GLUCAGON
IN VITRO
INSULIN
MICE
PANCREAS
PHENOTYPE
POLYMERASE CHAIN REACTION
SOMATOSTATIN
STEM CELLS
TRANSCRIPTION FACTORS