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Title: Functional heterogeneity of side population cells in skeletal muscle

Abstract

Skeletal muscle regeneration has been exclusively attributed to myogenic precursors, satellite cells. A stem cell-rich fraction referred to as side population (SP) cells also resides in skeletal muscle, but its roles in muscle regeneration remain unclear. We found that muscle SP cells could be subdivided into three sub-fractions using CD31 and CD45 markers. The majority of SP cells in normal non-regenerating muscle expressed CD31 and had endothelial characteristics. However, CD31{sup -}CD45{sup -} SP cells, which are a minor subpopulation in normal muscle, actively proliferated upon muscle injury and expressed not only several regulatory genes for muscle regeneration but also some mesenchymal lineage markers. CD31{sup -}CD45{sup -} SP cells showed the greatest myogenic potential among three SP sub-fractions, but indeed revealed mesenchymal potentials in vitro. These SP cells preferentially differentiated into myofibers after intramuscular transplantation in vivo. Our results revealed the heterogeneity of muscle SP cells and suggest that CD31{sup -}CD45{sup -} SP cells participate in muscle regeneration.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [2]
  1. Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo 187-8502 (Japan)
  2. Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo 187-8502 (Japan). E-mail: takeda@ncnp.go.jp
Publication Date:
OSTI Identifier:
20798847
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 341; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2006.01.037; PII: S0006-291X(06)00104-5; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; GENES; IN VITRO; IN VIVO; INJURIES; MUSCLES; REGENERATION; STEM CELLS

Citation Formats

Uezumi, Akiyoshi, Ojima, Koichi, Fukada, So-ichiro, Ikemoto, Madoka, Masuda, Satoru, Miyagoe-Suzuki, Yuko, and Takeda, Shin'ichi. Functional heterogeneity of side population cells in skeletal muscle. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2006.01.037.
Uezumi, Akiyoshi, Ojima, Koichi, Fukada, So-ichiro, Ikemoto, Madoka, Masuda, Satoru, Miyagoe-Suzuki, Yuko, & Takeda, Shin'ichi. Functional heterogeneity of side population cells in skeletal muscle. United States. doi:10.1016/j.bbrc.2006.01.037.
Uezumi, Akiyoshi, Ojima, Koichi, Fukada, So-ichiro, Ikemoto, Madoka, Masuda, Satoru, Miyagoe-Suzuki, Yuko, and Takeda, Shin'ichi. Fri . "Functional heterogeneity of side population cells in skeletal muscle". United States. doi:10.1016/j.bbrc.2006.01.037.
@article{osti_20798847,
title = {Functional heterogeneity of side population cells in skeletal muscle},
author = {Uezumi, Akiyoshi and Ojima, Koichi and Fukada, So-ichiro and Ikemoto, Madoka and Masuda, Satoru and Miyagoe-Suzuki, Yuko and Takeda, Shin'ichi},
abstractNote = {Skeletal muscle regeneration has been exclusively attributed to myogenic precursors, satellite cells. A stem cell-rich fraction referred to as side population (SP) cells also resides in skeletal muscle, but its roles in muscle regeneration remain unclear. We found that muscle SP cells could be subdivided into three sub-fractions using CD31 and CD45 markers. The majority of SP cells in normal non-regenerating muscle expressed CD31 and had endothelial characteristics. However, CD31{sup -}CD45{sup -} SP cells, which are a minor subpopulation in normal muscle, actively proliferated upon muscle injury and expressed not only several regulatory genes for muscle regeneration but also some mesenchymal lineage markers. CD31{sup -}CD45{sup -} SP cells showed the greatest myogenic potential among three SP sub-fractions, but indeed revealed mesenchymal potentials in vitro. These SP cells preferentially differentiated into myofibers after intramuscular transplantation in vivo. Our results revealed the heterogeneity of muscle SP cells and suggest that CD31{sup -}CD45{sup -} SP cells participate in muscle regeneration.},
doi = {10.1016/j.bbrc.2006.01.037},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 341,
place = {United States},
year = {2006},
month = {3}
}