Direct interaction between cohesin complex and DNA replication machinery

Journal Article · Fri Mar 17 04:00:00 EST 2006 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.01.029· OSTI ID:20798843

Ryu, Min-Jung ^[1]; Kim, Beom-Jun ^[1]; Lee, Jeong-Won ^[1]; Lee, Min-Woo ^[1]; Choi, Hyun-Kyung ^[1]; Kim, Seong-Tae ^[1]

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1. Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, 300 Chonchon-Dong, Changan-Ku, Suwon, Kyunggi-Do 440-746 (Korea, Republic of)

Structural maintenance of chromosome 1 (Smc1) is a multifunctional protein, which has been implicated in sister chromatid cohesion, DNA recombination and repair, and the activation of cell cycle checkpoints by ionizing radiation, ultraviolet light, and other genotoxic agents. In order to identify the proteins that interact with Smc1, we conducted the Tandem affinity purification (TAP) technique and analyzed the Smc1-interacting proteins via MALDI-TOF mass spectrometry. We identified minichromosome maintenance 7 (Mcm7), an essential component of the pre-replication complex, as a novel Smc1-interacting protein. Co-immunoprecipitation revealed an interaction occurring between Smc1 and Mcm7, both in vitro and in vivo. Using a GST pull-down assay, we determined that Smc1 interacts physically with Mcm7 via its N-terminal and hinge regions, and Mcm7 interacts with Smc1 via its middle region. Interestingly, we also discovered that Smc1 interacts with other DNA replication proteins, including Mcm6, RFC1, and DNA polymerase {alpha}. These results suggest that a functional link exists between the cohesin complex and DNA replication proteins.

• OSTI ID: 20798843
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 341; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
AFFINITY
CELL CYCLE
CHROMATIDS
CHROMOSOMES
DNA REPAIR
DNA REPLICATION
IN VITRO
IN VIVO
IONIZING RADIATIONS
MASS SPECTROSCOPY
PROTEINS
PURIFICATION
ULTRAVIOLET RADIATION