Glucose regulated proteins 78 and 75 bind to the receptor for hyaluronan mediated motility in interphase microtubules
- Department of Pathology, Osaka Medical College, 2-7, Takatsuki, Osaka 569-8686 (Japan)
- Biochemistry and Molecular Biology Laboratory, Aichi Prefectural College of Nursing and Health, Aichi 463-8502 (Japan)
- Department of Anatomy, Osaka Medical College, 2-7, Takatsuki, Osaka 569-8686 (Japan)
- Department of Medical Technology, Hirosaki University School of Health Sciences, Hirosaki 036-8560 (Japan)
The receptor for hyaluronan mediated motility (RHAMM), which is a hyaluronan-binding protein, is a centrosomal and microtubal protein. Here, we have identified two RHAMM-binding proteins, glucose regulated protein (GRP) 78 and GRP75, using co-immunoprecipitation analysis. These two proteins directly bound to glutathione-S-transferase-RHAMM fusion proteins. By double immunostaining, GRP78 and GRP75 colocalized with RHAMM in interphase microtubules, but were separated in mitotic spindles. Prevention of microtubule polymerization by TN-16 and vincristine sulfate induced RHAMM overexpression without a significant change in GRP78/75. Taken together, GRP78/75 and RHAMM complexes may stabilize microtubules in the interphase, associated with a downregulation of RHAMM. These results reveal a new biochemical activity of RHAMM.
- OSTI ID:
- 20798758
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 339, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2005.11.101; PII: S0006-291X(05)02625-2; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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