Downregulation of cyclin-dependent kinase inhibitor; p57{sup kip2}, is involved in the cell cycle progression of vascular smooth muscle cells
Journal Article
·
· Biochemical and Biophysical Research Communications
- Department of Cardiovascular Medicine, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638 (Japan)
- Division of Molecular Oncology, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815 (Japan)
Immature vascular smooth muscle cells (VSMCs) proliferate responding to extrinsic mitogens and accumulate in neointima after arterial injuries. Cell proliferation is positively regulated by cyclin/cyclin-dependent kinase (CDK) complex and negatively controlled by CDK inhibitors; CKIs such as p27{sup kip1} and p57{sup kip2}. In this study, embryonic rat thoracic aorta VSMCs; A10 were G0/G1 arrested by serum starvation, re-stimulated with serum, and harvested every four hours. Both CKIs co-expressed in quiescent VSMCs and rapidly diminished by stimulation. Protein level of p27{sup kip1} was regulated by both transcription and post-transcription, but that of p57{sup kip2} was mainly by post-transcription. Supplemental overexpression of p57{sup kip2} inhibited the activations of G1 cyclin/CDKs and subsequent hyperphosphorylations of all three retinoblastoma pocket proteins as well as G1/S transition of cell cycle. Our findings suggest that the downregulations of not only p27{sup kip1}, but also p57{sup kip2} responding to mitogenic stimulation, play key roles in the cell cycle progression of VSMCs.
- OSTI ID:
- 20793239
- Journal Information:
- Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 338; ISSN 0006-291X; ISSN BBRCA9
- Country of Publication:
- United States
- Language:
- English
Similar Records
CDK inhibitors, p21{sup Cip1} and p27{sup Kip1}, participate in cell cycle exit of mammalian cardiomyocytes
Cyclin D2 induces proliferation of cardiac myocytes and represses hypertrophy
Two inhibitory systems and CKIs regulate cell cycle exit of mammalian cardiomyocytes after birth
Journal Article
·
Thu Jan 16 23:00:00 EST 2014
· Biochemical and Biophysical Research Communications
·
OSTI ID:22242289
Cyclin D2 induces proliferation of cardiac myocytes and represses hypertrophy
Journal Article
·
Wed Mar 09 23:00:00 EST 2005
· Experimental Cell Research
·
OSTI ID:20717552
Two inhibitory systems and CKIs regulate cell cycle exit of mammalian cardiomyocytes after birth
Journal Article
·
Fri Oct 16 00:00:00 EDT 2015
· Biochemical and Biophysical Research Communications
·
OSTI ID:22592752