Human cytochrome c enters murine J774 cells and causes G{sub 1} and G{sub 2}/M cell cycle arrest and induction of apoptosis

Journal Article · Fri Dec 16 04:00:00 EST 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2005.1· OSTI ID:20793232

Hiraoka, Yoshinori ^[1]; Granja, Ana Teresa ^[1]; Fialho, Arsenio M ^[1]; Schlarb-Ridley, Beatrix G ^[1]; Das Gupta, Tapas K ^[2]; Chakrabarty, Ananda M ^[1]; Yamada, Tohru ^[3]

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1. Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago, IL (United States)
2. Department of Surgical Oncology, University of Illinois College of Medicine, Chicago, IL (United States)
3. Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago, IL (United States) and Department of Surgical Oncology, University of Illinois College of Medicine, Chicago, IL (United States)

Cytochrome c is well known as a carrier of electrons during respiration. Current evidence indicates that cytochrome c also functions as a major component of apoptosomes to induce apoptosis in eukaryotic cells as well as an antioxidant. More recently, a prokaryotic cytochrome c, cytochrome c {sub 551} from Pseudomonas aeruginosa, has been shown to enter in mammalian cells such as the murine macrophage-like J774 cells and causes inhibition of cell cycle progression. Much less is known about such functions by mammalian cytochromes c, particularly the human cytochrome c. We now report that similar to P. aeruginosa cytochrome c {sub 551}, the purified human cytochrome c protein can enter J774 cells and induce cell cycle arrest at the G{sub 1} to S phase, as well as at the G{sub 2}/M phase at higher concentrations. Unlike P. aeruginosa cytochrome c {sub 551} which had no effect on the induction of apoptosis, human cytochrome c induces significant apoptosis and cell death in J774 cells, presumably through inhibition of the cell cycle at the G{sub 2}/M phase. When incubated with human breast cancer MCF-7 and normal mammary epithelial cell line MCF-10A1 cells, human cytochrome c entered in both types of cells but induced cell death only in the normal MCF-10A1 cells. The ability of human cytochrome c to enter J774 cells was greatly reduced at 4 deg. C, suggesting energy requirement in the entry process.

• OSTI ID: 20793232
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 338; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANTIOXIDANTS
APOPTOSIS
CELL CYCLE
CYTOCHROMES
MACROPHAGES
MAMMARY GLANDS
NEOPLASMS
PSEUDOMONAS
RESPIRATION