Analysis of gene-expression profiles after gamma irradiation of normal human fibroblasts
- Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo (Japan) and Department of Radiation Oncology and Image-applied Therapy, Graduate School of Medicine, Kyoto University, Sakyo, Kyoto (Japan)
- Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo (Japan)
- Laboratory for Medical Informatics, SNP Research Center, Riken - Institute of Physical and Chemical Research, Yokohama Institute, Tsurumi (Japan)
- Department of Radiation Oncology and Image-applied Therapy, Graduate School of Medicine, Kyoto University, Sakyo, Kyoto (Japan)
Purpose: To understand comprehensive transcriptional profile of normal human fibroblast in response to irradiation. Methods and Materials: To identify genes whose expression is influenced by {gamma} radiation, we used a cDNA microarray to analyze expression of 23,000 genes in normal human fibroblasts at 7 timepoints (1, 3, 6, 12, 24, 48, and 72 hours) after 5 different doses (0.5, 2, 5, 15, and 50 Gy) of exposure. Results: Among the genes that showed altered expression patterns, some were already known to be regulated by irradiation, for instance ODC, EGR1, FGF2, PCNA, PKC, and several p53-target genes, including p53DINP1, BTG2, GADD45, and MDM2. The time course of each dose showed that from 350 to 600 genes were affected as to their expression; induction profiles characteristic to each dose were demonstrated. Of the total identified, only 89 genes were up-regulated; the vast majority was down-regulated over the 72-hour time course. We identified 21 genes that were distinctly induced by irradiation; 11 of them were functionally known, and 6 of those were p53-target genes. Conclusions: The results underscored the complexity of the transcriptional responses to irradiation, and the data should serve as a basis for global characterization of radiation-regulated genes and pathways.
- OSTI ID:
- 20788295
- Journal Information:
- International Journal of Radiation Oncology, Biology and Physics, Vol. 64, Issue 1; Other Information: DOI: 10.1016/j.ijrobp.2005.08.030; PII: S0360-3016(05)02395-3; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0360-3016
- Country of Publication:
- United States
- Language:
- English
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