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Title: Effects of garlic oil and two of its major organosulfur compounds, diallyl disulfide and diallyl trisulfide, on intestinal damage in rats injected with endotoxin

Abstract

Garlic and its active components are known to possess antioxidant and antiinflammatory effects. The present study investigated the effects of garlic oil and its organosulfur compounds on endotoxin-induced intestinal mucosal damage. Wistar rats received by gavage 50 or 200 mg/kg body weight garlic oil (GO), 0.5 mmol/kg body weight diallyl disulfide or diallyl trisulfide, or the vehicle (corn oil; 2 ml/kg body weight) every other day for 2 weeks before being injected with endotoxin (i.p., 5 mg/kg body weight). Control rats were administered with corn oil and were injected with sterile saline. Samples for the measurement of proinflammatory cytokines were collected 3 h after injection, and all other samples were collected 18 h after injection. The low dose of GO suppressed endotoxin-induced inducible nitric oxide synthase (iNOS) activity, ulceration, and apoptosis in the intestinal mucosa (P < 0.05). The high dose of GO significantly lowered the peripheral level of nitrate/nitrite and endotoxin-induced iNOS activity in the intestinal mucosa (P < 0.05) but worsened intestinal mucosal damage accompanied by elevated peripheral proinflammatory cytokines. Diallyl trisulfide but not diallyl disulfide showed similar toxic effect as that of high-dose GO. These results suggest the preventive effect and possible toxicity of garlic oil andmore » its organosulfur compounds in endotoxin-induced systemic inflammation and intestinal damage.« less

Authors:
 [1];  [1];  [1];  [1];  [2];  [1]
  1. Department of Nutrition, Chung Shan Medical University, No. 110, Sec. 1, Chien Kuo N. Road Taichung, Taiwan 402 (China)
  2. Graduate Institute of Food Science and Technology, Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, Taiwan (China)
Publication Date:
OSTI Identifier:
20783478
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 213; Journal Issue: 1; Other Information: DOI: 10.1016/j.taap.2005.08.008; PII: S0041-008X(05)00534-X; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIOXIDANTS; APOPTOSIS; BIOTIN; CORN OIL; DISULFIDES; GARLIC; INDIUM OXIDES; INFLAMMATION; LABELLING; LEAD SULFIDES; LYMPHOKINES; MUCOUS MEMBRANES; NITRATES; NITRIC OXIDE; NITRITES; PHOSPHATES; RATS; TOXICITY

Citation Formats

Chiang, Y -H, Jen, L -N, Su, H -Y, Lii, C -K, Sheen, L -Y, and Liu, C -T. Effects of garlic oil and two of its major organosulfur compounds, diallyl disulfide and diallyl trisulfide, on intestinal damage in rats injected with endotoxin. United States: N. p., 2006. Web. doi:10.1016/j.taap.2005.08.008.
Chiang, Y -H, Jen, L -N, Su, H -Y, Lii, C -K, Sheen, L -Y, & Liu, C -T. Effects of garlic oil and two of its major organosulfur compounds, diallyl disulfide and diallyl trisulfide, on intestinal damage in rats injected with endotoxin. United States. doi:10.1016/j.taap.2005.08.008.
Chiang, Y -H, Jen, L -N, Su, H -Y, Lii, C -K, Sheen, L -Y, and Liu, C -T. Mon . "Effects of garlic oil and two of its major organosulfur compounds, diallyl disulfide and diallyl trisulfide, on intestinal damage in rats injected with endotoxin". United States. doi:10.1016/j.taap.2005.08.008.
@article{osti_20783478,
title = {Effects of garlic oil and two of its major organosulfur compounds, diallyl disulfide and diallyl trisulfide, on intestinal damage in rats injected with endotoxin},
author = {Chiang, Y -H and Jen, L -N and Su, H -Y and Lii, C -K and Sheen, L -Y and Liu, C -T},
abstractNote = {Garlic and its active components are known to possess antioxidant and antiinflammatory effects. The present study investigated the effects of garlic oil and its organosulfur compounds on endotoxin-induced intestinal mucosal damage. Wistar rats received by gavage 50 or 200 mg/kg body weight garlic oil (GO), 0.5 mmol/kg body weight diallyl disulfide or diallyl trisulfide, or the vehicle (corn oil; 2 ml/kg body weight) every other day for 2 weeks before being injected with endotoxin (i.p., 5 mg/kg body weight). Control rats were administered with corn oil and were injected with sterile saline. Samples for the measurement of proinflammatory cytokines were collected 3 h after injection, and all other samples were collected 18 h after injection. The low dose of GO suppressed endotoxin-induced inducible nitric oxide synthase (iNOS) activity, ulceration, and apoptosis in the intestinal mucosa (P < 0.05). The high dose of GO significantly lowered the peripheral level of nitrate/nitrite and endotoxin-induced iNOS activity in the intestinal mucosa (P < 0.05) but worsened intestinal mucosal damage accompanied by elevated peripheral proinflammatory cytokines. Diallyl trisulfide but not diallyl disulfide showed similar toxic effect as that of high-dose GO. These results suggest the preventive effect and possible toxicity of garlic oil and its organosulfur compounds in endotoxin-induced systemic inflammation and intestinal damage.},
doi = {10.1016/j.taap.2005.08.008},
journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 1,
volume = 213,
place = {United States},
year = {2006},
month = {5}
}