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Title: The NADPH oxidase inhibitor apocynin (acetovanillone) induces oxidative stress

Abstract

Apocynin (acetovanillone) is often used as a specific inhibitor of NADPH oxidase. In N11 glial cells, apocynin induced, in a dose-dependent way, a significant increase of both malonyldialdehyde level (index of lipid peroxidation) and lactate dehydrogenase release (index of a cytotoxic effect). Apocynin evoked also, in a significant way, an increase of H{sub 2}O{sub 2} concentration and a decrease of the intracellular glutathione/glutathione disulfide ratio, accompanied by augmented efflux of glutathione and glutathione disulfide. Apocynin induced the activation of both pentose phosphate pathway and tricarboxylic acid cycle, which was blocked when the cells were incubated with glutathione together with apocynin. The cell incubation with glutathione prevented also the apocynin-induced increase of malonyldialdehyde generation and lactate dehydrogenase leakage. Apocynin exerted an oxidant effect also in a cell-free system: indeed, in aqueous solution, it evoked a faster oxidation of the thiols glutathione and dithiothreitol, and elicited the generation of reactive oxygen species, mainly superoxide anions. Our results suggest that apocynin per se can induce an oxidative stress and exert a cytotoxic effect in N11 cells and other cell types, and that some effects of apocynin in in vitro and in vivo experimental models should be interpreted with caution.

Authors:
 [1];  [1];  [1];  [2]
  1. Dipartimento di Genetica, Biologia e Biochimica, Universita di Torino, Via Santena 5/bis, 10126 Turin (Italy)
  2. Dipartimento di Genetica, Biologia e Biochimica, Universita di Torino, Via Santena 5/bis, 10126 Turin (Italy). E-mail: dario.ghigo@unito.it
Publication Date:
OSTI Identifier:
20783465
Resource Type:
Journal Article
Resource Relation:
Journal Name: Toxicology and Applied Pharmacology; Journal Volume: 212; Journal Issue: 3; Other Information: DOI: 10.1016/j.taap.2005.07.011; PII: S0041-008X(05)00426-6; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AQUEOUS SOLUTIONS; BIOLOGICAL STRESS; DISULFIDES; GLUCOSE; GLUTATHIONE; HYDROGEN PEROXIDE; IN VITRO; IN VIVO; LACTATE DEHYDROGENASE; LIPIDS; OXIDASES; OXIDATION; OXIDIZERS; PHOSPHATES; THIOLS

Citation Formats

Riganti, Chiara, Costamagna, Costanzo, Bosia, Amalia, and Ghigo, Dario. The NADPH oxidase inhibitor apocynin (acetovanillone) induces oxidative stress. United States: N. p., 2006. Web. doi:10.1016/j.taap.2005.07.011.
Riganti, Chiara, Costamagna, Costanzo, Bosia, Amalia, & Ghigo, Dario. The NADPH oxidase inhibitor apocynin (acetovanillone) induces oxidative stress. United States. doi:10.1016/j.taap.2005.07.011.
Riganti, Chiara, Costamagna, Costanzo, Bosia, Amalia, and Ghigo, Dario. Mon . "The NADPH oxidase inhibitor apocynin (acetovanillone) induces oxidative stress". United States. doi:10.1016/j.taap.2005.07.011.
@article{osti_20783465,
title = {The NADPH oxidase inhibitor apocynin (acetovanillone) induces oxidative stress},
author = {Riganti, Chiara and Costamagna, Costanzo and Bosia, Amalia and Ghigo, Dario},
abstractNote = {Apocynin (acetovanillone) is often used as a specific inhibitor of NADPH oxidase. In N11 glial cells, apocynin induced, in a dose-dependent way, a significant increase of both malonyldialdehyde level (index of lipid peroxidation) and lactate dehydrogenase release (index of a cytotoxic effect). Apocynin evoked also, in a significant way, an increase of H{sub 2}O{sub 2} concentration and a decrease of the intracellular glutathione/glutathione disulfide ratio, accompanied by augmented efflux of glutathione and glutathione disulfide. Apocynin induced the activation of both pentose phosphate pathway and tricarboxylic acid cycle, which was blocked when the cells were incubated with glutathione together with apocynin. The cell incubation with glutathione prevented also the apocynin-induced increase of malonyldialdehyde generation and lactate dehydrogenase leakage. Apocynin exerted an oxidant effect also in a cell-free system: indeed, in aqueous solution, it evoked a faster oxidation of the thiols glutathione and dithiothreitol, and elicited the generation of reactive oxygen species, mainly superoxide anions. Our results suggest that apocynin per se can induce an oxidative stress and exert a cytotoxic effect in N11 cells and other cell types, and that some effects of apocynin in in vitro and in vivo experimental models should be interpreted with caution.},
doi = {10.1016/j.taap.2005.07.011},
journal = {Toxicology and Applied Pharmacology},
number = 3,
volume = 212,
place = {United States},
year = {Mon May 01 00:00:00 EDT 2006},
month = {Mon May 01 00:00:00 EDT 2006}
}