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Title: Alveolar macrophages have a dual role in a rat model for trimellitic anhydride-induced occupational asthma

Abstract

Occupational exposure to low molecular weight chemicals, like trimellitic anhydride (TMA), can result in occupational asthma. Alveolar macrophages (AMs) are among the first cells to encounter inhaled compounds. These cells can produce many different mediators that have a putative role in asthma. In this study, we examined the role of AMs in lung function and airway inflammation of rats exposed to TMA. Female Brown Norway rats were sensitized by dermal application of TMA or received vehicle alone on days 0 and 7. One day before challenge, rats received intratracheally either empty or clodronate-containing liposomes to deplete the lungs of AMs. On day 21, all rats were challenged by inhalation of TMA in air. Lung function parameters were measured before, during, within 1 h after, and 24 h after challenge. IgE levels and parameters of inflammation and tissue damage were assessed 24 h after challenge. Sensitization with TMA led to decreased lung function parameters during and within 1 h after challenge as compared to non-sensitized rats. AM depletion alleviated the TMA-induced drop in lung function parameters and induced a faster recovery compared to sham-depleted TMA-sensitized rats. It also decreased the levels of serum IgE 24 h after challenge, but did notmore » affect the sensitization-dependent increase in lung lavage fluid IL-6 and tissue TNF-{alpha} levels. In contrast, AM depletion augmented the TMA-induced tissue damage and inflammation 24 h after challenge. AMs seem to have a dual role in this model for TMA-induced occupational asthma since they potentiate the immediate TMA-induced decrease in lung function but tended to dampen the TMA-induced inflammatory reaction 24 h later.« less

Authors:
 [1];  [1];  [1];  [2];  [3];  [3];  [1];  [4];  [5]
  1. Department of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA, Utrecht (Netherlands)
  2. Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht (Netherlands)
  3. TNO Nutrition and Food Research Institute, Zeist (Netherlands)
  4. (Netherlands)
  5. Department of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA, Utrecht (Netherlands). E-mail: p.a.j.henricks@pharm.uu.nl
Publication Date:
OSTI Identifier:
20783426
Resource Type:
Journal Article
Resource Relation:
Journal Name: Toxicology and Applied Pharmacology; Journal Volume: 211; Journal Issue: 1; Other Information: DOI: 10.1016/j.taap.2005.05.012; PII: S0041-008X(05)00298-X; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANHYDRIDES; ASTHMA; DAMAGE; INFLAMMATION; INHALATION; LIPOSOMES; LUNGS; MACROPHAGES; MOLECULAR WEIGHT; OCCUPATIONAL EXPOSURE; RATS

Citation Formats

Valstar, Dingena L., Schijf, Marcel A., Nijkamp, Frans P., Storm, Gert, Arts, Josje H.E., Kuper, C. Frieke, Bloksma, Nanne, Faculty of Biology, Utrecht University, Utrecht, and Henricks, Paul A.J. Alveolar macrophages have a dual role in a rat model for trimellitic anhydride-induced occupational asthma. United States: N. p., 2006. Web. doi:10.1016/j.taap.2005.05.012.
Valstar, Dingena L., Schijf, Marcel A., Nijkamp, Frans P., Storm, Gert, Arts, Josje H.E., Kuper, C. Frieke, Bloksma, Nanne, Faculty of Biology, Utrecht University, Utrecht, & Henricks, Paul A.J. Alveolar macrophages have a dual role in a rat model for trimellitic anhydride-induced occupational asthma. United States. doi:10.1016/j.taap.2005.05.012.
Valstar, Dingena L., Schijf, Marcel A., Nijkamp, Frans P., Storm, Gert, Arts, Josje H.E., Kuper, C. Frieke, Bloksma, Nanne, Faculty of Biology, Utrecht University, Utrecht, and Henricks, Paul A.J. Wed . "Alveolar macrophages have a dual role in a rat model for trimellitic anhydride-induced occupational asthma". United States. doi:10.1016/j.taap.2005.05.012.
@article{osti_20783426,
title = {Alveolar macrophages have a dual role in a rat model for trimellitic anhydride-induced occupational asthma},
author = {Valstar, Dingena L. and Schijf, Marcel A. and Nijkamp, Frans P. and Storm, Gert and Arts, Josje H.E. and Kuper, C. Frieke and Bloksma, Nanne and Faculty of Biology, Utrecht University, Utrecht and Henricks, Paul A.J.},
abstractNote = {Occupational exposure to low molecular weight chemicals, like trimellitic anhydride (TMA), can result in occupational asthma. Alveolar macrophages (AMs) are among the first cells to encounter inhaled compounds. These cells can produce many different mediators that have a putative role in asthma. In this study, we examined the role of AMs in lung function and airway inflammation of rats exposed to TMA. Female Brown Norway rats were sensitized by dermal application of TMA or received vehicle alone on days 0 and 7. One day before challenge, rats received intratracheally either empty or clodronate-containing liposomes to deplete the lungs of AMs. On day 21, all rats were challenged by inhalation of TMA in air. Lung function parameters were measured before, during, within 1 h after, and 24 h after challenge. IgE levels and parameters of inflammation and tissue damage were assessed 24 h after challenge. Sensitization with TMA led to decreased lung function parameters during and within 1 h after challenge as compared to non-sensitized rats. AM depletion alleviated the TMA-induced drop in lung function parameters and induced a faster recovery compared to sham-depleted TMA-sensitized rats. It also decreased the levels of serum IgE 24 h after challenge, but did not affect the sensitization-dependent increase in lung lavage fluid IL-6 and tissue TNF-{alpha} levels. In contrast, AM depletion augmented the TMA-induced tissue damage and inflammation 24 h after challenge. AMs seem to have a dual role in this model for TMA-induced occupational asthma since they potentiate the immediate TMA-induced decrease in lung function but tended to dampen the TMA-induced inflammatory reaction 24 h later.},
doi = {10.1016/j.taap.2005.05.012},
journal = {Toxicology and Applied Pharmacology},
number = 1,
volume = 211,
place = {United States},
year = {Wed Feb 15 00:00:00 EST 2006},
month = {Wed Feb 15 00:00:00 EST 2006}
}