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Title: Diagnostic approaches for viruses and prions in stem cell banks

Abstract

Some stem cell lines may contain an endogenous virus or can be contaminated with exogenous viruses (even of animal origin) and may secrete viral particles or express viral antigens on their surface. Moreover, certain biotechnological products (e.g. bovine fetal serum, murine feeder cells) may contain prion particles. Viral and prion contamination of cell cultures and 'feeder' cells, which is a common risk in all biotechnological products derived from the cell lines, is the most challenging and potentially serious outcome to address, due to the difficulty involved in virus and prion detection and the potential to cause serious disease in recipients of these cell products. Stem cell banks should introduce adequate quality assurance programs like the microbiological control program and can provide researchers with valuable support in the standardization and safety of procedures and protocols used for the viral and prion testing and in validation programs to assure the quality and safety of the cells.

Authors:
 [1];  [2];  [2]
  1. Stem Cell Bank of Andalucia (Spanish Central Node), Hospital Universitario Virgen de las Nieves, Avda Fuerzas Armadas, 2, 18014 Granada (Spain). E-mail: fernancobo@fundacionhvn.org
  2. Stem Cell Bank of Andalucia (Spanish Central Node), Hospital Universitario Virgen de las Nieves, Avda Fuerzas Armadas, 2, 18014 Granada (Spain)
Publication Date:
OSTI Identifier:
20779472
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 347; Journal Issue: 1; Other Information: DOI: 10.1016/j.virol.2005.11.026; PII: S0042-6822(05)00772-5; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIGENS; BIOTECHNOLOGY; CATTLE; CELL CULTURES; HEALTH HAZARDS; QUALITY ASSURANCE; STEM CELLS; THERAPY; VIRUSES

Citation Formats

Cobo, Fernando, Talavera, Paloma, and Concha, Angel. Diagnostic approaches for viruses and prions in stem cell banks. United States: N. p., 2006. Web. doi:10.1016/J.VIROL.2005.1.
Cobo, Fernando, Talavera, Paloma, & Concha, Angel. Diagnostic approaches for viruses and prions in stem cell banks. United States. doi:10.1016/J.VIROL.2005.1.
Cobo, Fernando, Talavera, Paloma, and Concha, Angel. Thu . "Diagnostic approaches for viruses and prions in stem cell banks". United States. doi:10.1016/J.VIROL.2005.1.
@article{osti_20779472,
title = {Diagnostic approaches for viruses and prions in stem cell banks},
author = {Cobo, Fernando and Talavera, Paloma and Concha, Angel},
abstractNote = {Some stem cell lines may contain an endogenous virus or can be contaminated with exogenous viruses (even of animal origin) and may secrete viral particles or express viral antigens on their surface. Moreover, certain biotechnological products (e.g. bovine fetal serum, murine feeder cells) may contain prion particles. Viral and prion contamination of cell cultures and 'feeder' cells, which is a common risk in all biotechnological products derived from the cell lines, is the most challenging and potentially serious outcome to address, due to the difficulty involved in virus and prion detection and the potential to cause serious disease in recipients of these cell products. Stem cell banks should introduce adequate quality assurance programs like the microbiological control program and can provide researchers with valuable support in the standardization and safety of procedures and protocols used for the viral and prion testing and in validation programs to assure the quality and safety of the cells.},
doi = {10.1016/J.VIROL.2005.1},
journal = {Virology},
number = 1,
volume = 347,
place = {United States},
year = {Thu Mar 30 00:00:00 EST 2006},
month = {Thu Mar 30 00:00:00 EST 2006}
}
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