Maturation of the viral core enhances the fusion of HIV-1 particles with primary human T cells and monocyte-derived macrophages

Journal Article · Wed Mar 15 04:00:00 EST 2006 · Virology

DOI:https://doi.org/10.1016/J.VIROL.2005.1· OSTI ID:20779471

Jiyang, Jiang ^[1]; Aiken, Christopher ^[1]

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1. Department of Microbiology and Immunology, Vanderbilt University School of Medicine, A-5301 Medical Center North, Nashville, TN 37232-2363 (United States)

HIV-1 infection requires fusion of viral and cellular membranes in a reaction catalyzed by the viral envelope proteins gp120 and gp41. We recently reported that efficient HIV-1 particle fusion with target cells is linked to maturation of the viral core by an activity of the gp41 cytoplasmic domain. Here, we show that maturation enhances the fusion of a variety of recombinant viruses bearing primary and laboratory-adapted Env proteins with primary human CD4{sup +} T cells. Overall, HIV-1 fusion was more dependent on maturation for viruses bearing X4-tropic envelope proteins than for R5-tropic viruses. Fusion of HIV-1 with monocyte-derived macrophages was also dependent on particle maturation. We conclude that the ability to couple fusion to particle maturation is a common feature of HIV-1 Env proteins and may play an important role during HIV-1 replication in vivo.

• OSTI ID: 20779471
• Journal Information: Virology, Journal Name: Virology Journal Issue: 2 Vol. 346; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

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