Factors influencing subcellular localization of the human papillomavirus L2 minor structural protein

Journal Article · Sun Feb 05 04:00:00 EST 2006 · Virology

DOI:https://doi.org/10.1016/J.VIROL.2005.0· OSTI ID:20779457

Kieback, Elisa ^[1]; Mueller, Martin ^[2]

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1. Max-Delbrueck-Center for Molecular Medicine, Berlin (Germany)
2. Deutsches Krebsforschungszentrum, Program Infection and Cancer, Heidelberg (Germany)

Two structural proteins form the capsids of papillomaviruses. The major structural protein L1 is the structural determinant of the capsids and is present in 360 copies arranged in 72 pentamers. The minor structural protein L2 is estimated to be present in twelve copies per capsid. Possible roles for L2 in interaction with cell surface receptors and in virion uptake have been suggested. As previously reported, L2 localizes in subnuclear domains identified as nuclear domain 10 (ND10). As it was demonstrated that L2 is able to recruit viral and cellular proteins to ND10, a possible role for L2 as a mediator in viral assembly has been proposed. In this study, we determined factors influencing the localization of L2 at ND10. Under conditions of moderate L2 expression level and in the absence of heterologous viral components, we observed that, in contrast to previous reports, L2 is mainly distributed homogeneously throughout the nucleus. L2, however, is recruited to ND10 at a higher expression level or in the presence of viral components derived from vaccinia virus or from Semliki Forest virus. We observed that translocation of L2 to ND10 is not a concentration-dependent accumulation but rather seems to be triggered by yet unidentified cellular factors. In contrast to HPV 11 and 16 L2, the HPV 18 L2 protein seems to require L1 for efficient nuclear accumulation.

• OSTI ID: 20779457
• Journal Information: Virology, Journal Name: Virology Journal Issue: 1 Vol. 345; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
FORESTS
HUMAN POPULATIONS
RECEPTORS
TRANSLOCATION
UPTAKE
VACCINIA VIRUS