Hydroxyl-radical-dependent DNA damage by ambient particulate matter from contrasting sampling locations

Journal Article · Mon May 15 04:00:00 EDT 2006 · Environmental Research

DOI:https://doi.org/10.1016/j.envres.2005.09.005· OSTI ID:20775309

Tingming, Shi ^[1]; Duffin, Rodger ^[1]; Borm, Paul J.A. ^[1]; Hui, Li ^[1]; Weishaupt, Christel ^[1]; Schins, Roel P.F. ^[1]

---

1. Institut fuer Umweltmedizinische Forschung an der Heinrich-Heine University Duesseldorf gGmbH, auf'm Hennekamp 50, D-40225 Duesseldorf (Germany)

Exposure to ambient particulate matter (PM) has been reported to be associated with increased respiratory, cardiovascular, and malignant lung disease. Previously we have shown that PM can induce oxidative DNA damage in A549 human lung epithelial cells. The aims of the present study were to investigate the variability of the DNA-damaging properties of PM sampled at different locations and times and to relate the observed effects to the hydroxyl-radical ({center_dot}OH)-generating activities of these samples. Weekly samples of coarse (10-2.5 {mu}m) and fine (<2.5 {mu}m) PM from four sites (Nordrheim Westfalen, Germany) were analyzed for hydrogen-peroxide-dependent {center_dot}OH formation using electron paramagnetic resonance and formation of 8-hydroxydeoxyguanosine (8-OHdG) in calf thymus DNA using an immuno-dot-blot assay. DNA strand breakage by fine PM in A549 human lung epithelial cells was quantified using the alkaline comet assay. Both PM size distribution fractions elicited {center_dot}OH generation and 8-OHdG formations in calf thymus DNA. Significantly higher {center_dot}OH generation was observed for PM sampled at urban/industrial locations and for coarse PM. Samples of fine PM also caused DNA strand breakage in A549 cells and this damage could be prevented using the hydroxyl-radical scavengers 5,5-dimethyl-1-pyrroline-N-oxide and dimethyl sulfoxide. The observed DNA strand breakage appeared to correlate with the hydroxyl-radical-generating capacities of the PM samples but with different profiles for rural versus urban/industrial samples. In conclusion, when considered at equal mass, {center_dot}OH formation of PM shows considerable variability with regard to the sampling location and time and is correlated with its ability to cause DNA damage.

• OSTI ID: 20775309
• Journal Information: Environmental Research, Journal Name: Environmental Research Journal Issue: 1 Vol. 101; ISSN ENVRAL; ISSN 0013-9351
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CALVES
DMSO
DNA
DNA DAMAGES
ELECTRON SPIN RESONANCE
HYDROGEN PEROXIDE
HYDROXYL RADICALS
LUNGS
OXIDES
RESPIRATORY SYSTEM DISEASES
SAMPLING
THYMUS