Structure-Driven Liquid Microjunction Surface-Sampling Probe Mass Spectrometry

Kertesz, Vilmos; Khalid, Muneeba; Retterer, Scott T.; Cahill, John F.

doi:10.1021/acs.analchem.3c02370

Structure-Driven Liquid Microjunction Surface-Sampling Probe Mass Spectrometry

Journal Article · Fri Sep 22 00:00:00 EDT 2023 · Analytical Chemistry

DOI:https://doi.org/10.1021/acs.analchem.3c02370· OSTI ID:2076197

^[1]; Khalid, Muneeba ^[1]; ^[2]; ^[1]

Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)

The rhizosphere is the narrow region of soil surrounding the roots of plants that is influenced by root exudates, root secretions, and associated microbial communities. This region is crucial to plant growth and development and plays a critical role in nutrient uptake, disease resistance, and soil transformation. Understanding the function of exogenous compounds in the rhizosphere starts with determining the spatiotemporal distribution of these molecular components. Using liquid microjunction surface-sampling probe mass spectrometry (LMJ-SSP-MS) and microfluidic devices with attached microporous membranes enables in situ, nondisruptive, and nondestructive spatiotemporal measurement of exogenous compounds from plant roots. However, long imaging times (>2 h) can negatively affect plant heath and limit temporal studies. Here, we present a novel strategy to optimize the number and location of sampling sites on these microporous membrane-covered microfluidic devices. In conclusion, this novel, “structure-driven” sampling workflow takes into consideration the channel structure of the microfluidic device to maximize sampling from the channels and minimize acquisition time (~4× less time in some cases while providing similar chemical image accuracy), thus reducing stress on plants during in situ LMJ-SSP-MS analysis.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 2076197

Journal Information:: Analytical Chemistry, Journal Name: Analytical Chemistry Journal Issue: 39 Vol. 95; ISSN 0003-2700

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Structure-Driven Liquid Microjunction Surface-Sampling Probe Mass Spectrometry

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