Antibody response is required for protection from Theiler's virus-induced encephalitis in C57BL/6 mice in the absence of CD8{sup +} T cells

Journal Article · Thu Sep 15 04:00:00 EDT 2005 · Virology

DOI:https://doi.org/10.1016/j.virol.2005.06.028· OSTI ID:20729128

Kang, B -S ^[1]; Palma, Joann P ^[1]; Lyman, Michael A ^[1]; Dal Canto, Mauro ^[2]; Kim, Byung S ^[3]

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1. Department of Microbiology-Immunology, Northwestern University, Medical School, 303 East Chicago Avenue, Chicago, IL 60611 (United States)
2. Department of Pathology, Northwestern University, Medical School, 303 East Chicago Avenue, Chicago, IL 60611 (United States)
3. Department of Microbiology-Immunology, Northwestern University, Medical School, 303 East Chicago Avenue, Chicago, IL 60611 (United States) and Department of Pathology, Northwestern University, Medical School, 303 East Chicago Avenue, Chicago, IL 60611 (United States)

Intracerebral infection of susceptible mice with Theiler's murine encephalomyelitis virus (TMEV) induces immune-mediated demyelinating disease and this system serves as a relevant infectious model for human multiple sclerosis. It was previously shown that {beta}{sub 2}M-deficient C57BL/6 mice lacking functional CD8{sup +} T cells display increased viral persistence and enhanced susceptibility to TMEV-induced demyelination, and yet the majority of mice are free of clinical signs. To understand the mechanisms involved in this general resistance of C57BL/6 mice in the absence of CTL responses, mice ({mu}MT) deficient in the B-cell compartment lacking membrane IgM molecules were treated with anti-CD8 antibody and then infected with TMEV. Although little difference in the proliferative responses of peripheral T cells to UV-inactivated TMEV and the resistance to demyelinating disease was observed between virus-infected {mu}MT and control B6 mice, the levels of CD4{sup +} T cells were higher in the CNS of {mu}MT mice. However, after treatment with anti-CD8 antibody, 100% of the mice displayed clinical gray matter disease and prolonged viral persistence in {mu}MT mice, while only 10% of B6 mice showed clinical symptoms and very low viral persistence. Transfusion of sera from TMEV-infected B6 mice into anti-CD8 antibody-treated {mu}MT mice partially restored resistance to virus-induced encephalitis. These results indicate that the early anti-viral antibody response is also important in the protection from TMEV-induced encephalitis particularly in the absence of CD8{sup +} T cells.

• OSTI ID: 20729128
• Journal Information: Virology, Journal Name: Virology Journal Issue: 1 Vol. 340; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANTIBODIES
CENTRAL NERVOUS SYSTEM
ENCEPHALITIS
MICE
SYMPTOMS
VIRUSES