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Title: Distinct protease pathways control cell shape and apoptosis in v-src-transformed quail neuroretina cells

Abstract

Intracellular proteases play key roles in cell differentiation, proliferation and apoptosis. In nerve cells, little is known about their relative contribution to the pathways which control cell physiology, including cell death. Neoplastic transformation of avian neuroretina cells by p60 {sup v-src} tyrosine kinase results in dramatic morphological changes and deregulation of apoptosis. To identify the proteases involved in the cellular response to p60 {sup v-src}, we evaluated the effect of specific inhibitors of caspases, calpains and the proteasome on cell shape changes and apoptosis induced by p60 {sup v-src} inactivation in quail neuroretina cells transformed by tsNY68, a thermosensitive strain of Rous sarcoma virus. We found that the ubiquitin-proteasome pathway is recruited early after p60 {sup v-src} inactivation and is critical for morphological changes, whereas caspases are essential for cell death. This study provides evidence that distinct intracellular proteases are involved in the control of the morphology and fate of v-src-transformed cells.

Authors:
 [1]; ;  [1];  [2];  [3]
  1. IBCP, UMR 5086 CNRS/Universite Claude Bernard, IFR 128, 7 passage du Vercors, F69367, Lyon cedex 07 (France)
  2. Dynamique Cellulaire, Ecole Pratique des Hautes Etudes, IFRT 130, UMR CNRS 5525 Universite Joseph Fourier, 38706 La Tronche cedex (France)
  3. IBCP, UMR 5086 CNRS/Universite Claude Bernard, IFR 128, 7 passage du Vercors, F69367, Lyon cedex 07 (France). E-mail: g.gillet@ibcp.fr
Publication Date:
OSTI Identifier:
20717688
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 311; Journal Issue: 1; Other Information: DOI: 10.1016/j.yexcr.2005.09.001; PII: S0014-4827(05)00413-1; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; CELL DIFFERENTIATION; MICROTUBULES; MORPHOLOGICAL CHANGES; MORPHOLOGY; NERVE CELLS; ONCOGENIC VIRUSES; PHYSIOLOGY; RETINA; TRANSFORMATIONS; TYROSINE

Citation Formats

Neel, Benjamin D., Aouacheria, Abdel, Nouvion, Anne-Laure, Ronot, Xavier, and Gillet, Germain. Distinct protease pathways control cell shape and apoptosis in v-src-transformed quail neuroretina cells. United States: N. p., 2005. Web. doi:10.1016/j.yexcr.2005.09.001.
Neel, Benjamin D., Aouacheria, Abdel, Nouvion, Anne-Laure, Ronot, Xavier, & Gillet, Germain. Distinct protease pathways control cell shape and apoptosis in v-src-transformed quail neuroretina cells. United States. doi:10.1016/j.yexcr.2005.09.001.
Neel, Benjamin D., Aouacheria, Abdel, Nouvion, Anne-Laure, Ronot, Xavier, and Gillet, Germain. Tue . "Distinct protease pathways control cell shape and apoptosis in v-src-transformed quail neuroretina cells". United States. doi:10.1016/j.yexcr.2005.09.001.
@article{osti_20717688,
title = {Distinct protease pathways control cell shape and apoptosis in v-src-transformed quail neuroretina cells},
author = {Neel, Benjamin D. and Aouacheria, Abdel and Nouvion, Anne-Laure and Ronot, Xavier and Gillet, Germain},
abstractNote = {Intracellular proteases play key roles in cell differentiation, proliferation and apoptosis. In nerve cells, little is known about their relative contribution to the pathways which control cell physiology, including cell death. Neoplastic transformation of avian neuroretina cells by p60 {sup v-src} tyrosine kinase results in dramatic morphological changes and deregulation of apoptosis. To identify the proteases involved in the cellular response to p60 {sup v-src}, we evaluated the effect of specific inhibitors of caspases, calpains and the proteasome on cell shape changes and apoptosis induced by p60 {sup v-src} inactivation in quail neuroretina cells transformed by tsNY68, a thermosensitive strain of Rous sarcoma virus. We found that the ubiquitin-proteasome pathway is recruited early after p60 {sup v-src} inactivation and is critical for morphological changes, whereas caspases are essential for cell death. This study provides evidence that distinct intracellular proteases are involved in the control of the morphology and fate of v-src-transformed cells.},
doi = {10.1016/j.yexcr.2005.09.001},
journal = {Experimental Cell Research},
number = 1,
volume = 311,
place = {United States},
year = {Tue Nov 15 00:00:00 EST 2005},
month = {Tue Nov 15 00:00:00 EST 2005}
}