Myogenic program induction in mature fat tissue (with MyoD expression)

Journal Article · Mon Aug 15 04:00:00 EDT 2005 · Experimental Cell Research

DOI:https://doi.org/10.1016/j.yexcr.2005.03.038· OSTI ID:20717641

Kocaefe, Y C ^[1]; Israeli, D ^[2]; Ozguc, M ^[3]; Danos, O ^[2]; Garcia, L ^[2]

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1. Genethon CNRS UMR8115 1 bis rue de l'Internationale 91002 Evry cedex (France) and Hacettepe University, Faculty of Medicine, Department of Medical Biology, Ankara (Turkey)
2. Genethon CNRS UMR8115 1 bis rue de l'Internationale 91002 Evry cedex (France)
3. Hacettepe University, Faculty of Medicine, Department of Medical Biology, Ankara (Turkey)

MyoD exerts a master transcriptional control over the myogenic differentiation cascade. Here, we study different approaches to induce myogenic transdifferentiation in mature adipocytes utilizing MyoD gene transfer. Organotypic cultures of fat tissue and a long-term culture of in vitro differentiated adipocytes deduced that MyoD provoked morphological changes in mature adipocytes that can be summarized as loss of fat content, acquisition of a fusiform shape and eventual fusion with committed neighbor cells. In vivo, MyoD gene transfer into rat interscapular and inguinal fat pads demonstrated that while structural proteins of muscle lineage were expressed, they co-existed with specific adipocyte proteins. Expression of these proteins diminished over time likewise the fat content. The transdifferentiation process initiated by MyoD did not require cell cycle progression and was well tolerated by the fully differentiated and mature adipocytes.

• OSTI ID: 20717641
• Journal Information: Experimental Cell Research, Journal Name: Experimental Cell Research Journal Issue: 2 Vol. 308; ISSN 0014-4827; ISSN ECREAL
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL CYCLE
FATS
GENES
IN VITRO
IN VIVO
MORPHOLOGICAL CHANGES
MUSCLES
PROTEINS
RATS