Anti-inflammatory effects of polyunsaturated fatty acids in THP-1 cells
- Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA (United States)
- Department of Nutritional Sciences, Pennsylvania State University, University Park, PA (United States)
- Department of Veterinary Science, Pennsylvania State University, University Park, PA (United States)
- DuPont Nutrition and Health, Newark, DE (United States)
- Department of Biobehavioral Health, Pennsylvania State University, University Park, PA (United States)
The effects of linoleic acid (LA), {alpha}-linolenic acid (ALA), and docosahexaenoic acid (DHA) were compared to that of palmitic acid (PA), on inflammatory responses in human monocytic THP-1 cells. When cells were pre-incubated with fatty acids for 2-h and then stimulated with lipopolysaccharide for 24-h in the presence of fatty acids, secretion of interleukin (IL)-6, IL-1{beta}, and tumor necrosis factor-{alpha} (TNF{alpha}) was significantly decreased after treatment with LA, ALA, and DHA versus PA (P < 0.01 for all); ALA and DHA elicited more favorable effects. These effects were comparable to those for 15-deoxy-{delta}{sup 12,14}-prostaglandin J2 (15d-PGJ2) and were dose-dependent. In addition, LA, ALA, and DHA decreased IL-6, IL-1{beta}, and TNF{alpha} gene expression (P < 0.05 for all) and nuclear factor (NF)-{kappa}B DNA-binding activity, whereas peroxisome proliferator-activated receptor-{gamma} (PPAR{gamma}) DNA-binding activity was increased. The results indicate that the anti-inflammatory effects of polyunsaturated fatty acids may be, in part, due to the inhibition of NF-{kappa}B activation via activation of PPAR{gamma}.
- OSTI ID:
- 20713405
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 336, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2005.08.204; PII: S0006-291X(05)01933-9; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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