Negative regulation of {beta}-catenin/Tcf signaling by naringenin in AGS gastric cancer cell

Journal Article · Fri Sep 30 04:00:00 EDT 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.07.146· OSTI ID:20710994

Lee, Ju Hyung ^[1]; Park, Chi Hoon ^[1]; Jung, Kyung Chae ^[1]; Rhee, Ho Sung ^[1]; Yang, Chul Hak ^[1]

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1. Division of Chemistry and Molecular Engineering, Seoul National University, Seoul 151-742 (Korea, Republic of)

Functional activation of {beta}-catenin/Tcf signaling plays an important role in early events in carcinogenesis. We examined the effect of naringenin against {beta}-catenin/Tcf signaling in gastric cancer cells. Reporter gene assay showed that naringenin inhibited {beta}-catenin/Tcf signaling efficiently. In addition, the inhibition of {beta}-catenin/Tcf signaling by naringenin in HEK293 cells transiently transfected with constitutively mutant {beta}-catenin gene, whose product is not phosphorylated by GSK3{beta}, indicates that its inhibitory mechanism was related to {beta}-catenin itself or downstream components. To investigate the precise inhibitory mechanism, we performed immunofluorescence, Western blot, and EMSA. As a result, our data revealed that the {beta}-catenin distribution and the levels of nuclear {beta}-catenin and Tcf-4 proteins were unchanged after naringenin treatment. Moreover, the binding activities of Tcf complexes to consensus DNA were not affected by naringenin. Taken together, these data suggest that naringenin inhibits {beta}-catenin/Tcf signaling in gastric cancer with unknown mechanisms.

• OSTI ID: 20710994
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 335; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CARCINOGENESIS
DNA
GENE REGULATION
INHIBITION
MUTANTS
NEOPLASMS
PROTEINS