Electron capture dissociation mass spectrometry in characterization of post-translational modifications

Journal Article · Fri Aug 19 04:00:00 EDT 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.05.138· OSTI ID:20710908

Bakhtiar, Ray ^[1]; Ziqiang, Guan ^[2]

---

1. Merck Research Laboratories, Rahway, NJ 07065 (United States)
2. Department of Biochemistry, Duke University Medical Center, P.O. Box 3711, Durham, NC 27710 (United States)

Electron capture dissociation (ECD) represents a significant advance in tandem mass spectrometry for the identification and characterization of post-translational modifications (PTMs) of polypeptides. In comparison with the conventional fragmentation techniques, such as collisionally induced dissociation and infrared multi-photon dissociation, ECD provides more extensive sequence fragments, while allowing the labile modifications to remain intact during backbone fragmentation. This unique attribute offers ECD as an attractive alternative for detection and localization of PTMs. The success and rapid adoption of ECD recently led to the culmination of The 1st International Uppsala Symposium on Electron Capture Dissociation of Biomolecules and Related Phenomena (October 19-22, 2003, Stockholm, Sweden). Herein, we present a general overview of the ECD technique as well as selected applications in characterization of post-translationally modified polypeptides.

• OSTI ID: 20710908
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 334; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
AMINO ACID SEQUENCE
DISSOCIATION
ELECTRON CAPTURE
FOURIER TRANSFORMATION
IONIZATION
MASS SPECTROSCOPY
MULTI-PHOTON PROCESSES
POLYPEPTIDES