Evaluation of adriamycin nephropathy by an in vivo electron paramagnetic resonance

Journal Article · Fri Jul 01 04:00:00 EDT 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.04.134· OSTI ID:20710827

Oteki, Takaaki ^[1]; Nagase, Sohji ^[1]; Yokoyama, Hidekatsu ^[2]; Ohya, Hiroaki ^[2]; Akatsuka, Takao ^[2]; Tada, Mika ^[3]; Ueda, Atsushi ^[1]; Hirayama, Aki ^[1]; koyama, Akio ^[1]

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1. Pathophysiology of Renal Diseases, Medical Sciences for Control of Pathological Processes, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki (Japan)
2. Yamagata Promotional Organization for Industrial Technology, Yamagata (Japan)
3. New Industry Creation Hatchery Center, University of Tohoku, Miyagi (Japan)

A rat model for human minimal change nephropathy was obtained by the intravenous injection of adriamycin (ADR) at 5 mg/kg. By using an in vivo electron paramagnetic resonance (EPR) spectrometer operating at 700 MHz, the temporal changes in signal intensities of a nitroxide radical, 4-hydroxyl-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPOL), in the kidneys of rats with ADR nephropathy were investigated. The decay rate of the EPR signal intensity obtained in the kidney is indicative of the renal reducing ability. It was found that the reducing ability in the kidney declined on the 7th day after ADR administration and recovered after the 14th day. Impairment of the reducing ability occurred before the appearance of continuous urinary protein. The in vitro EPR study showed that this impairment of in vivo renal reducing ability is related to impairment of the reducing ability in the mitochondria.

• OSTI ID: 20710827
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 332; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
DOXORUBICIN
ELECTRON SPIN RESONANCE
EPR SPECTROMETERS
EVALUATION
HYDROXIDES
IN VITRO
IN VIVO
INTRAVENOUS INJECTION
KIDNEYS
MITOCHONDRIA
PROTEINS
RATS