Nuclear translocation of phospholipase C-zeta, an egg-activating factor, during early embryonic development

Journal Article · Fri May 13 04:00:00 EDT 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.03.032· OSTI ID:20709178

Sone, Yoshie ^[1]; Ito, Masahiko ^[1]; Shirakawa, Hideki ^[1]; Shikano, Tomohide ^[1]; Takeuchi, Hiroyuki ^[2]; Kinoshita, Katsuyuki ^[2]; Miyazaki, Shunichi ^[1]

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1. Department of Physiology, Tokyo Women's Medical University School of Medicine, Tokyo 162-8666 (Japan)
2. Department of Obstetrics and Gynecology, Juntendo University School of Medicine, Tokyo 113-8241 (Japan)

Phospholipase C-zeta (PLC{zeta}), a strong candidate of the egg-activating sperm factor, causes intracellular Ca{sup 2+} oscillations and egg activation, and is subsequently accumulated into the pronucleus (PN), when expressed in mouse eggs by injection of RNA encoding PLC{zeta}. Changes in the localization of expressed PLC{zeta} were investigated by tagging with a fluorescent protein. PLC{zeta} began to translocate into the PN formed at 5-6 h after RNA injection and increased there. Observation in the same embryo revealed that PLC{zeta} in the PN dispersed to the cytoplasm upon nuclear envelope breakdown and translocated again into the nucleus after cleavage. The dynamics was found in the second mitosis as well. When RNA was injected into fertilization-originated 1-cell embryos or blastomere(s) of 2-8-cell embryos, the nuclear localization of expressed PLC{zeta} was recognized in every embryo up to blastocyst. Thus, PLC{zeta} exhibited alternative cytoplasm/nucleus localization during development. This supports the view that the sperm factor could control cell cycle-dependent generation of Ca{sup 2+} oscillations in early embryogenesis.

• OSTI ID: 20709178
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 330; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CALCIUM IONS
CELL CYCLE
CLEAVAGE
CYTOPLASM
EMBRYOS
FERTILIZATION
MICE
MITOSIS
ONTOGENESIS
OSCILLATIONS
PROTEINS
RNA
SPERMATOZOA
TRANSLOCATION