Basic calcium phosphate crystal-induced Egr-1 expression stimulates mitogenesis in human fibroblasts
- Department of Biomedical Engineering, University of Miami, Coral Gablek, FL 33146 (United States)
- Division of Rheumatology and Immunology, Department of Medicine, University of Miami School of Medicine, Miami, FL 33101 (United States)
- Department of Biomedical Engineering, University of Miami, Coral Gablek, FL 33146 (United States) and Division of Rheumatology and Immunology, Department of Medicine, University of Miami School of Medicine, Miami, FL 33101 (United States) and Research Service and the Geriatric Research, Education, and Clinical Center, Veterans Affairs Medical Center, Miami, FL 33125 (United States)
Previously, we have reported that basic calcium phosphate (BCP) crystals stimulate mitogenesis and synthesis of matrix metalloproteinases in cultured human foreskin and synovial fibroblasts. However, the detailed mechanisms involved are still unclear. In the present study, using RT-PCR and Egr-1 promoter analysis we showed that BCP crystals could stimulate early growth response gene Egr-1 transcription through a PKC{alpha}-dependent p44/p42 MAPK pathway. Using a retrovirus gene expression system (Clontech) to overexpress Egr-1 in human fibroblast BJ-1 cells resulted in promotion of mitogenesis measured either by MTT cell proliferation analysis or by direct cell counting. The results demonstrate that Egr-1 may play a key role in mediating BCP crystal-induced synovial fibroblast mitogenesis.
- OSTI ID:
- 20709176
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 330, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2005.03.036; PII: S0006-291X(05)00526-7; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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