In vivo imaging of oxidative stress in the kidney of diabetic mice and its normalization by angiotensin II type 1 receptor blocker

Journal Article · Fri May 06 04:00:00 EDT 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.02.174· OSTI ID:20709166

Sonta, Toshiyo ^[1]; Inoguchi, Toyoshi ^[1]; Matsumoto, Shingo ^[2]; Yasukawa, Keiji ^[2]; Inuo, Mieko ^[1]; Tsubouchi, Hirotaka ^[1]; Sonoda, Noriyuki ^[1]; Kobayashi, Kunihisa ^[1]; Utsumi, Hideo ^[2]; Nawata, Hajime ^[1]

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1. Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka (Japan)
2. Department of Bio-function Science, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka (Japan)

This study was undertaken to evaluate oxidative stress in the kidney of diabetic mice by electron spin resonance (ESR) imaging technique. Oxidative stress in the kidney was evaluated as organ-specific reducing activity with the signal decay rates of carbamoyl-PROXYL probe using ESR imaging. The signal decay rates were significantly faster in corresponding image pixels of the kidneys of streptozotocin-induced diabetic mice than in those of controls. This technique further demonstrated that administration of angiotensin II type 1 receptor blocker (ARB), olmesartan (5 mg/kg), completely restored the signal decay rates in the diabetic kidneys to control values. In conclusion, this study provided for the first time the in vivo evidence for increased oxidative stress in the kidneys of diabetic mice and its normalization by ARB as evaluated by ESR imaging. This technique would be useful as a means of further elucidating the role of oxidative stress in diabetic nephropathy.

• OSTI ID: 20709166
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 330; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANGIOTENSIN
ELECTRON SPIN RESONANCE
IN VIVO
KIDNEYS
MICE
RECEPTORS
STREPTOZOCIN