{sup 19}F NMR measurements of NO production in hypertensive ISIAH and OXYS rats
- Institute of Chemical Kinetics and Combustion, Novosibirsk 630090 (Russian Federation)
- Institute of Cytology and Genetics, Novosibirsk 630090 (Russian Federation)
- International Tomography Centre, Novosibirsk 630090 (Russian Federation)
- Dorothy M. Davis Heart and Lung Research Institute, Ohio State University, Columbus, OH 43210 (United States)
Recently we demonstrated the principal possibility of application of {sup 19}F NMR spin-trapping technique for in vivo {sup {center_dot}}NO detection [Free Radic. Biol. Med. 36 (2004) 248]. In the present study, we employed this method to elucidate the significance of {sup {center_dot}}NO availability in animal models of hypertension. In vivo {sup {center_dot}}NO-induced conversion of the hydroxylamine of the fluorinated nitronyl nitroxide (HNN) to the hydroxylamine of the iminonitroxide (HIN) in hypertensive ISIAH and OXYS rat strains and normotensive Wistar rat strain was measured. Significantly lower HIN/HNN ratios were measured in the blood of the hypertensive rats. The NMR data were found to positively correlate with the levels of nitrite/nitrate evaluated by Griess method and negatively correlate with the blood pressure. In comparison with other traditionally used methods {sup 19}F NMR spectroscopy allows in vivo evaluation of {sup {center_dot}}NO production and provides the basis for in vivo {sup {center_dot}}NO imaging.
- OSTI ID:
- 20709164
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 330, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2005.02.166; PII: S0006-291X(05)00458-4; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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