Modeling single molecule detection probabilities in microdroplets. Final report
Numerical tools for modeling the fluorescence collected from a single molecule within a microsphere as a function of it`s position and orientation, the size of the droplet, the numerical aperture of the lens, the detection geometry, type of illumination, and the linewidth of the emitting molecule, are described.
- Research Organization:
- Army Research Lab., White Sands Missile Range, NM (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- AI05-95OR22401
- OSTI ID:
- 206995
- Report Number(s):
- DOE/OR/22401--T1; ON: DE96008232
- Country of Publication:
- United States
- Language:
- English
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