X-ray and neutron reflectivity study of solid-supported lipid membranes prepared by spin coating

Journal Article · Wed Dec 01 04:00:00 UTC 2004 · Journal of Applied Physics

DOI:https://doi.org/10.1063/1.1814412· OSTI ID:20658116

Generosi, Johanna; Castellano, Carlo; Pozzi, Daniela; Castellano, Agostina Congiu; Felici, Roberto; Natali, Francesca; Fragneto, Giovanna ^[1]

---

1. Dipartimento di Fisica and Istituto Nazionale per la Fisica della Materia, Universita 'La Sapienza', P.le Aldo Moro 2, 00185 Rome (Italy)

We present a study of x-ray synchrotron radiation and neutron reflectivity on solid-supported lipid membranes prepared by spin coating. This technique has the advantage of allowing the control of the number of lipid layers by varying the deposition parameters. The experiments were performed on the cationic lipid 1,2-dioleoyl-3-trimethylammonium-propane chloride salt (DOTAP), the neutral lipid 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), the lipid mixture (DOTAP-DOPC), and the complex (DOTAP-DOPC/DNA) deposited on wafers. Only single neutral lipids or lipid-peptide mixtures were deposited on solid substrate using the spin coating technique and characterized. Results on the structure of the deposited lipid layers indicate that DNA contributes to the order in the lipoplexes.

• OSTI ID: 20658116
• Journal Information: Journal of Applied Physics, Journal Name: Journal of Applied Physics Journal Issue: 11 Vol. 96; ISSN JAPIAU; ISSN 0021-8979
• Country of Publication: United States
• Language: English

Related Subjects

36 MATERIALS SCIENCE
CHLORIDES
DEPOSITION
DNA
LAYERS
LIPIDS
MEMBRANES
MIXTURES
NEUTRON DIFFRACTION
NEUTRONS
PEPTIDES
PROPANE
REFLECTION
REFLECTIVITY
SALTS
SPIN
SUBSTRATES
SYNCHROTRON RADIATION
THIN FILMS