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Title: Analysis of the interaction between respiratory syncytial virus and lipid-rafts in Hep2 cells during infection

Abstract

The assembly of respiratory syncytial virus (RSV) in lipid-rafts was examined in Hep2 cells. Confocal and electron microscopy showed that during RSV assembly, the cellular distribution of the complement regulatory proteins, decay accelerating factor (CD55) and CD59, changes and high levels of these cellular proteins are incorporated into mature virus filaments. The detergent-solubility properties of CD55, CD59, and the RSV fusion (F) protein were found to be consistent with each protein being located predominantly within lipid-raft structures. The levels of these proteins in cell-released virus were examined by immunoelectronmicroscopy and found to account for between 5% and 15% of the virus attachment (G) glycoprotein levels. Collectively, our findings suggest that an intimate association exists between RSV and lipid-raft membranes and that significant levels of these host-derived raft proteins, such as those regulating complement activation, are subsequently incorporated into the envelope of mature virus particles.

Authors:
 [1];  [2];  [1];  [1];  [3];  [4]
  1. MRC Virology Unit, Institute of Virology, Glasgow G11 5JR (United Kingdom)
  2. Institute of Cell and Molecular Biology, Biological Sciences EM Facility, University of Edinburgh, Edinburgh EH9 3JN (United Kingdom)
  3. IBLS Division of Virology, University of Glasgow, Institute of Virology, Glasgow G11 5JR (United Kingdom)
  4. MRC Virology Unit, Institute of Virology, Glasgow G11 5JR (United Kingdom). E-mail: r.sugrue@vir.gla.ac.uk
Publication Date:
OSTI Identifier:
20634874
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 327; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2004.06.038; PII: S0042-6822(04)00396-4; Copyright (c) 2004 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; DECAY; DETERGENTS; ELECTRON MICROSCOPY; FILAMENTS; GLYCOPROTEINS; LIPIDS; PARTICLES; VIRUSES

Citation Formats

Brown, Gaie, Jeffree, Chris E., McDonald, Terence, McL Rixon, Helen W., Aitken, James D., and Sugrue, Richard J. Analysis of the interaction between respiratory syncytial virus and lipid-rafts in Hep2 cells during infection. United States: N. p., 2004. Web. doi:10.1016/j.virol.2004.06.038.
Brown, Gaie, Jeffree, Chris E., McDonald, Terence, McL Rixon, Helen W., Aitken, James D., & Sugrue, Richard J. Analysis of the interaction between respiratory syncytial virus and lipid-rafts in Hep2 cells during infection. United States. doi:10.1016/j.virol.2004.06.038.
Brown, Gaie, Jeffree, Chris E., McDonald, Terence, McL Rixon, Helen W., Aitken, James D., and Sugrue, Richard J. Fri . "Analysis of the interaction between respiratory syncytial virus and lipid-rafts in Hep2 cells during infection". United States. doi:10.1016/j.virol.2004.06.038.
@article{osti_20634874,
title = {Analysis of the interaction between respiratory syncytial virus and lipid-rafts in Hep2 cells during infection},
author = {Brown, Gaie and Jeffree, Chris E. and McDonald, Terence and McL Rixon, Helen W. and Aitken, James D. and Sugrue, Richard J.},
abstractNote = {The assembly of respiratory syncytial virus (RSV) in lipid-rafts was examined in Hep2 cells. Confocal and electron microscopy showed that during RSV assembly, the cellular distribution of the complement regulatory proteins, decay accelerating factor (CD55) and CD59, changes and high levels of these cellular proteins are incorporated into mature virus filaments. The detergent-solubility properties of CD55, CD59, and the RSV fusion (F) protein were found to be consistent with each protein being located predominantly within lipid-raft structures. The levels of these proteins in cell-released virus were examined by immunoelectronmicroscopy and found to account for between 5% and 15% of the virus attachment (G) glycoprotein levels. Collectively, our findings suggest that an intimate association exists between RSV and lipid-raft membranes and that significant levels of these host-derived raft proteins, such as those regulating complement activation, are subsequently incorporated into the envelope of mature virus particles.},
doi = {10.1016/j.virol.2004.06.038},
journal = {Virology},
number = 2,
volume = 327,
place = {United States},
year = {Fri Oct 01 00:00:00 EDT 2004},
month = {Fri Oct 01 00:00:00 EDT 2004}
}