Irradiation combined with SU5416: Microvascular changes and growth delay in a human xenograft glioblastoma tumor line

Journal Article · Tue Feb 01 04:00:00 EST 2005 · International Journal of Radiation Oncology, Biology and Physics

DOI:https://doi.org/10.1016/j.ijrobp.2004.09.063· OSTI ID:20633061

Schuuring, Janneke ^[1]; Bussink, Johan ^[2]; Bernsen, Hans ^[3]; Peeters, Wenny ^[3]; Kogel, Albert J. van der ^[3]

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1. Department of NeurologyUniversity Medical Center, Nijmegen (Netherlands)
2. Department of Radiation Oncology, University Medical Center, Nijmegen (Netherlands)
3. Department ofRadiation Oncology, University Medical Center, Nijmegen (Netherlands)

Purpose: The combination of irradiation and the antiangiogenic compound SU5416 was tested and compared with irradiation alone in a human glioblastoma tumor line xenografted in nude mice. The aim of this study was to monitor microenvironmental changes and growth delay. Methods and materials: A human glioblastoma xenograft tumor line was implanted in nude mice. Irradiations consisted of 10 Gy or 20 Gy with and without SU5416. Several microenvironmental parameters (tumor cell hypoxia, tumor blood perfusion, vascular volume, and microvascular density) were analyzed after imunohistochemical staining. Tumor growth delay was monitored for up to 200 days after treatment. Results: SU5416, when combined with irradiation, has an additive effect over treatment with irradiation alone. Analysis of the tumor microenvironment showed a decreased vascular density during treatment with SU5416. In tumors regrowing after reaching only a partial remission, vascular characteristics normalized shortly after cessation of SU5416. However, in tumors regrowing after reaching a complete remission, permanent microenvironmental changes and an increase of tumor necrosis with a subsequent slower tumor regrowth was found. Conclusions: Permanent vascular changes were seen after combined treatment resulting in complete remission. Antiangiogenic treatment with SU5416 when combined with irradiation has an additive effect over treatment with irradiation or antiangiogenic treatment alone.

• OSTI ID: 20633061
• Journal Information: International Journal of Radiation Oncology, Biology and Physics, Journal Name: International Journal of Radiation Oncology, Biology and Physics Journal Issue: 2 Vol. 61; ISSN IOBPD3; ISSN 0360-3016
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ANOXIA
BLOOD
GLIOMAS
IRRADIATION
MICE
NECROSIS
RADIOTHERAPY
TUMOR CELLS