{alpha}-Actinin-2, a cytoskeletal protein, binds to angiogenin

Journal Article · Fri Apr 08 04:00:00 EDT 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.01.158· OSTI ID:20630924

Huajun, Hu ^[1]; Xiangwei, Gao ^[1]; Yishan, Sun ^[1]; Jiliang, Zhou ^[2]; Min, Yang ^[2]; Zhengping, Xu ^[3]

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1. Research Center for Environmental Genomics, Zhejiang University School of Medicine, Hangzhou 310031 (China)
2. Department of Public Health, Zhejiang University School of Medicine, Hangzhou 310031 (China)
3. Research Center for Environmental Genomics, Zhejiang University School of Medicine, Hangzhou 310031 (China) and Department of Public Health, Zhejiang University School of Medicine, Hangzhou 310031 (China)

Angiogenin is an angiogenic factor which is involved in tumorigenesis. However, no particular intracellular protein is known to interact directly with angiogenin. In the present study, we reported the identification of {alpha}-actinin-2, an actin-crosslinking protein, as a potential angiogenin-interacting partner by yeast two-hybrid screening. This interaction was confirmed by different approaches. First, angiogenin was pulled down together with His-tagged {alpha}-actinin-2 by Ni{sup 2+}-agarose resins. Second, {alpha}-actinin-2 was coimmunoprecipitated with angiogenin by anti-angiogenin monoclonal antibody. Third, the in vivo interaction of these two proteins was revealed by fluorescence resonance energy transfer analysis. Since members of {alpha}-actinin family play pivotal roles in cell proliferation, migration, and invasion, the interaction between {alpha}-actinin-2 and angiogenin may underline one possible mechanism of angiogenin in angiogenesis. Our finding presents the first evidence of an interaction of a cytosolic protein with angiogenin, which might be a novel interference target for anti-angiogenesis and anti-tumor therapy.

• OSTI ID: 20630924
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 329; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ACTIN
CELL PROLIFERATION
CROSS-LINKING
ENERGY TRANSFER
FLUORESCENCE
HYBRIDIZATION
IN VIVO
MONOCLONAL ANTIBODIES
NEOPLASMS
NICKEL IONS
RESINS
THERAPY
YEASTS