Multiple functions of the vacuolar sorting protein Ccz1p in Saccharomyces cerevisiae

Journal Article · Fri Apr 01 04:00:00 EST 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.01.107· OSTI ID:20630907

Hoffman-Sommer, Marta ^[1]; Migdalski, Andrzej ^[1]; Rytka, Joanna ^[1]; Kucharczyk, Roza ^[1]

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1. Institute of Biochemistry and Biophysics PAS, Pawinskiego 5A, 02-106 Warsaw (Poland)

The CCZ1 (YBR131w) gene encodes a protein required for fusion of various transport intermediates with the vacuole. Ccz1p, in a complex with Mon1p, is a close partner of Ypt7p in the processes of fusion of endosomes to vacuoles and homotypic vacuole fusion. In this work, we exploited the Ca{sup 2+}-sensitivity of the ccz1{delta} mutant to identify genes specifically interacting with CCZ1, basing on functional multicopy suppression of calcium toxicity. The presented results indicate that Ccz1p functions in the cell either in association with Mon1p and Ypt7p in fusion at the vacuolar membrane, or-separately-with Arl1p at early steps of vacuolar transport. We also show that suppression of calcium toxicity by the calcium pumps Pmr1p and Pmc1p is restricted only to the subset of mutants defective in vacuole morphology. The mechanisms of Ca{sup 2+}-pump-mediated suppression also differ from each other, since the action of Pmr1p, but not Pmc1p, appears to require Arl1p function.

• OSTI ID: 20630907
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 329; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CALCIUM IONS
GENES
INHIBITION
MORPHOLOGY
MUTANTS
PROTEINS
SACCHAROMYCES CEREVISIAE
SENSITIVITY
TOXICITY