Fathead minnow vitellogenin: Complementary DNA sequence and messenger RNA and protein expression after 17{beta}-estradiol treatment
Journal Article
·
· Environmental Toxicology and Chemistry
Induction of vitellogenin (VTG) in oviparous animals has been proposed as a sensitive indicator of environmental contaminants that activate the estrogen receptor. In the present study, a sensitive ribonuclease protection assay (RPA) for VTG messenger RNA (mRNA) was developed for the fathead minnow (Pimephales promelas), a species proposed for routine endocrine-disrupting chemical (EDC) screening. The utility of this method was compared with an enzyme-linked immunosorbent assay (ELISA) specific for fathead minnow VTG protein. Assessment of the two methods included kinetic characterization of the plasma VTG protein and hepatic VTG mRNA levels in male fathead minnows following intraperitoneal injections of 17{beta}-estradiol (E2) at two dose levels (0.5, 5.0 mg/kg). Initial plasma E2 concentrations were elevated in a dose-dependent manner but returned to normal levels within 2 d. Lover VTG mRNA was detected within 4 h, reached a maximum around 48 h, and returned to normal levels in about 6 d. Plasma VTG protein was detectable within 16 h of treatment reached maximum levels at about 72 h. and remained near these maximum levels for at least 18 d. While the RPA was about 1,000 times more sensitive than the ELISA, the ELISA appears superior for routine screening tests. The ELISA method is relatively simple to perform and, because males lack a clearance mechanism for VTG, the protein remains at relatively high concentrations in the plasma for an extended period of time. As part of the development of the RPA, the complementary DNA (cDNA) sequence for fathead minnow VTG was determined and the deduced amino acid sequence compared with VTG sequences for other fish species.
- Research Organization:
- Environmental Protection Agency, Duluth, MN (US)
- OSTI ID:
- 20080483
- Journal Information:
- Environmental Toxicology and Chemistry, Journal Name: Environmental Toxicology and Chemistry Journal Issue: 4 Vol. 19; ISSN ETOCDK; ISSN 0730-7268
- Country of Publication:
- United States
- Language:
- English
Similar Records
Method for Detection and Quantitation of Fathead Minnow Vitellogenin (Vtg) by Liquid Chromatography and Matrix Assisted Laser Desorption/ Ionization Mass Spectrometry
Expression of two vitellogenin genes (vg1 and vg3) in fathead minnow (Pimephales promelas) liver in response to exposure to steroidal estrogens and androgens.
Effects of adrenergic agents on the expression of zebrafish (Danio rerio) vitellogenin Ao1
Journal Article
·
Thu Mar 10 23:00:00 EST 2005
· Aquatic Toxicology
·
OSTI ID:859978
Expression of two vitellogenin genes (vg1 and vg3) in fathead minnow (Pimephales promelas) liver in response to exposure to steroidal estrogens and androgens.
Journal Article
·
Tue Feb 28 23:00:00 EST 2006
· Ecotoxicology and Environmental Safety, 63(3):337-342
·
OSTI ID:882952
Effects of adrenergic agents on the expression of zebrafish (Danio rerio) vitellogenin Ao1
Journal Article
·
Wed Jul 01 00:00:00 EDT 2009
· Toxicology and Applied Pharmacology
·
OSTI ID:21272588