Novel genetic tools improve Penicillium expansum patulin synthase production in Aspergillus niger
Since the first CRISPR (clustered regularly interspaced short palindromic repeats)‐Cas (CRISPR‐associated) system was developed for creating double‐stranded DNA breaks, it has been adapted and improved for different biotechnological applications. In this issue of The FEBS Journal , Arentshorst et al . developed a novel approach to enhance transgene expression of a specific protein, patulin synthase (PatE) from Penicillium expansum , in the important industrial filamentous fungus Aspergillus niger . Their technique involved the disruption of selected genes with counter‐effects on targeted protein production and simultaneous integration of glucoamylase landing sites into the disrupted gene locus such as protease regulator ( prtT ) in an ATP‐dependent DNA helicase II subunit 1 ( kusA or ku70 ) ‐ deletion strain. Multiple copies of the PatE transgene expression cassette were introduced by CRISPR‐Cas9‐mediated insertion. The purified PatE was further used for structural and functional studies, and the technique laid the foundation for elevating the overall production of various proteins or chemicals in those industrially important fungi.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Organization:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Office of Sustainable Transportation. Bioenergy Technologies Office (BETO); USDOE Office of Science (SC), Biological and Environmental Research (BER)
- Grant/Contract Number:
- AC05-76RL01830; AC02-05CH11231; NL0030038
- OSTI ID:
- 2007684
- Alternate ID(s):
- OSTI ID: 2204436; OSTI ID: 2305380
- Report Number(s):
- PNNL-SA-190084
- Journal Information:
- Federation of European Biochemical Societies (FEBS) Journal, Journal Name: Federation of European Biochemical Societies (FEBS) Journal Vol. 290 Journal Issue: 21; ISSN 1742-464X
- Publisher:
- Wiley-BlackwellCopyright Statement
- Country of Publication:
- United Kingdom
- Language:
- English
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