Isolation of adherent polycyclic aromatic hydrocarbon (PAH)-degrading bacteria using PAH-sorbing carriers
Journal Article
·
· Applied and Environmental Microbiology
Two different procedures were compared to isolate polycyclic aromatic hydrocarbon (PAH)-utilizing bacteria from PAH-contaminated soil and sludge samples, i.e., (i) shaken enrichment cultures in liquid mineral medium in which PAHs were supplied as crystals and (ii) a new method in which PAH degraders were enriched on and recovered from hydrophobic membranes containing sorbed PAHs. Both techniques were successful, but selected from the same source different bacterial strains able to grow on PAHs as the sole source of carbon and energy. The liquid enrichment mainly selected for Sphingomonas spp., whereas the membrane method exclusively led to the selection of Mycobacterium spp. Furthermore, in separate membrane enrichment set-ups with different membrane types, three repetitive extragenic palindromic PCR-related Mycobacterium strains were recovered. The new Mycobactereium isolates were strongly hydrophobic and displayed the capacity to adhere strongly to different surfaces. One strain, Mycobacterium sp. LB501T, displayed an unusual combination of high adhesion efficiency and an extremely high negative charge. This strain may represent a new bacterial species as suggested by 16S rRNA gene sequence analysis. These results indicate that the provision of hydrophobic sorbents containing sorbed PAHs in the enrichment procedure discriminated in favor of certain bacterial characteristics. The new isolation method is appropriate to select for adherent PAH-degrading bacteria, which might be useful to biodegrade sorbed PAHs in soils and sludge.
- Research Organization:
- Vlaamse Instelling voor Technologisch Onderzoek, Mol (BE)
- OSTI ID:
- 20075757
- Journal Information:
- Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 5 Vol. 66; ISSN AEMIDF; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
Similar Records
Aerobic mineralization of sorbed biphenyl by a microbial consortium
Bioremediation of Petroleum and Radiological Contaminated Soils at the Savannah River Site: Laboratory to Field Scale Applications
Molecular detection, isolation, and physiological characterization of functionally dominant phenol-degrading bacteria in activated sludge
Thesis/Dissertation
·
Fri Dec 31 23:00:00 EST 1993
·
OSTI ID:39163
Bioremediation of Petroleum and Radiological Contaminated Soils at the Savannah River Site: Laboratory to Field Scale Applications
Conference
·
Mon Jun 07 00:00:00 EDT 2004
·
OSTI ID:825210
Molecular detection, isolation, and physiological characterization of functionally dominant phenol-degrading bacteria in activated sludge
Journal Article
·
Sat Oct 31 23:00:00 EST 1998
· Applied and Environmental Microbiology
·
OSTI ID:302269