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Title: Early life-stage mortality in zebrafish (Danio rerio) following maternal exposure to polychlorinated biphenyls and estrogen

Abstract

In the present study, specific polychlorinated biphenyl (PCB) congeners were examined for embryo and early life stage mortality in zebrafish (Danio rerio). A set of eight PCBs and two hydroxylated PCBs and 17{beta}-estradiol were tested. Of the compounds tested, 4{prime}-OH-PCB30 (hydroxylated polychlorinated biphenyl) and PCB104 were found to be highly toxic to embryos following maternal exposure and transfer to the oocyte. It was also observed that 17{beta}-estradiol exposure resulted in a high incidence of embryo mortality. Analysis of estrogen receptor levels during embryonic development showed increased mRNA (ribonucleic acid) levels from the 1K stage to 50% epiboly. Following injection of the different compounds, the estrogen receptor mRNA levels were also analyzed in adult male fish to determine if there was a correlation between embryo mortality and estrogenicity of the studied PCBs. The two PCBs that were highly embryo toxic were observed to be estrogenic.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Umeaa Univ. (SE)
OSTI Identifier:
20062544
Alternate Identifier(s):
OSTI ID: 20062544
Resource Type:
Journal Article
Journal Name:
Environmental Toxicology and Chemistry
Additional Journal Information:
Journal Volume: 19; Journal Issue: 6; Other Information: PBD: Jun 2000; Journal ID: ISSN 0730-7268
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; WATER POLLUTION; BIOLOGICAL INDICATORS; POLYCHLORINATED BIPHENYLS; FISHES; TOXICITY; ESTRADIOL; AGE DEPENDENCE

Citation Formats

Westerlund, L., Billsson, K., Andersson, P.L., Tysklind, M., and Olsson, P.E. Early life-stage mortality in zebrafish (Danio rerio) following maternal exposure to polychlorinated biphenyls and estrogen. United States: N. p., 2000. Web. doi:10.1897/1551-5028(2000)019<1582:ELSMIZ>2.3.CO;2.
Westerlund, L., Billsson, K., Andersson, P.L., Tysklind, M., & Olsson, P.E. Early life-stage mortality in zebrafish (Danio rerio) following maternal exposure to polychlorinated biphenyls and estrogen. United States. doi:10.1897/1551-5028(2000)019<1582:ELSMIZ>2.3.CO;2.
Westerlund, L., Billsson, K., Andersson, P.L., Tysklind, M., and Olsson, P.E. Thu . "Early life-stage mortality in zebrafish (Danio rerio) following maternal exposure to polychlorinated biphenyls and estrogen". United States. doi:10.1897/1551-5028(2000)019<1582:ELSMIZ>2.3.CO;2.
@article{osti_20062544,
title = {Early life-stage mortality in zebrafish (Danio rerio) following maternal exposure to polychlorinated biphenyls and estrogen},
author = {Westerlund, L. and Billsson, K. and Andersson, P.L. and Tysklind, M. and Olsson, P.E.},
abstractNote = {In the present study, specific polychlorinated biphenyl (PCB) congeners were examined for embryo and early life stage mortality in zebrafish (Danio rerio). A set of eight PCBs and two hydroxylated PCBs and 17{beta}-estradiol were tested. Of the compounds tested, 4{prime}-OH-PCB30 (hydroxylated polychlorinated biphenyl) and PCB104 were found to be highly toxic to embryos following maternal exposure and transfer to the oocyte. It was also observed that 17{beta}-estradiol exposure resulted in a high incidence of embryo mortality. Analysis of estrogen receptor levels during embryonic development showed increased mRNA (ribonucleic acid) levels from the 1K stage to 50% epiboly. Following injection of the different compounds, the estrogen receptor mRNA levels were also analyzed in adult male fish to determine if there was a correlation between embryo mortality and estrogenicity of the studied PCBs. The two PCBs that were highly embryo toxic were observed to be estrogenic.},
doi = {10.1897/1551-5028(2000)019<1582:ELSMIZ>2.3.CO;2},
journal = {Environmental Toxicology and Chemistry},
issn = {0730-7268},
number = 6,
volume = 19,
place = {United States},
year = {2000},
month = {6}
}