Evaluation of quantitative synchrotron radiation micro-X-ray fluorescence in rice grain

Limmer, Matt A.; Webb, Samuel M.; Seyfferth, Angelia L.

doi:10.1107/s1600577523000747

Evaluation of quantitative synchrotron radiation micro-X-ray fluorescence in rice grain

Journal Article · Wed Feb 15 00:00:00 EST 2023 · Journal of Synchrotron Radiation (Online)

DOI:https://doi.org/10.1107/s1600577523000747· OSTI ID:2004589

^[1]; ^[2]; Seyfferth, Angelia L. ^[3]

Univ. of Delaware, Newark, DE (United States); SLAC
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)
Univ. of Delaware, Newark, DE (United States)

Concentrations of nutrients and contaminants in rice grain affect human health, specifically through the localization and chemical form of elements. Methods to spatially quantify the concentration and speciation of elements are needed to protect human health and characterize elemental homeostasis in plants. Here, an evaluation was carried out using quantitative synchrotron radiation microprobe X-ray fluorescence (SR-µXRF) imaging by comparing average rice grain concentrations of As, Cu, K, Mn, P, S and Zn measured with rice grain concentrations from acid digestion and ICP-MS analysis for 50 grain samples. Better agreement was found between the two methods for high-Z elements. Regression fits between the two methods allowed quantitative concentration maps of the measured elements. These maps revealed that most elements were concentrated in the bran, although S and Zn permeated into the endosperm. Arsenic was highest in the ovular vascular trace (OVT), with concentrations approaching 100 mg kg^–1 in the OVT of a grain from a rice plant grown in As-contaminated soil. Quantitative SR-µXRF is a useful approach for comparison across multiple studies but requires careful consideration of sample preparation and beamline characteristics.

View Accepted Manuscript (DOE)

Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 2004589

Journal Information:: Journal of Synchrotron Radiation (Online), Journal Name: Journal of Synchrotron Radiation (Online) Journal Issue: 2 Vol. 30; ISSN 1600-5775

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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73 NUCLEAR PHYSICS AND RADIATION PHYSICS
SR-µXRF
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rice bran
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Evaluation of quantitative synchrotron radiation micro-X-ray fluorescence in rice grain

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