2,4,6-Trinitrotoluene reduction by carbon monoxide dehydrogenase from Clostridium thermoaceticum

Journal Article · Wed Mar 01 04:00:00 EST 2000 · Applied and Environmental Microbiology

DOI:https://doi.org/10.1128/AEM.66.4.1474-1478.2000· OSTI ID:20026718

Huang, S; Lindahl, P A; Wang, C; Bennett, G N; Rudolph, F B; Hughes, J B

Purified CO dehydrogenase (CODH) from Clostridium thermoaceticum catalyzed the transformation of 2,4,6-trinitrotoluene (TNT). The intermediates and reduced products of TNT transformation were separated and appear to be identical to the compounds formed by C. acetobutylicum, namely, 2-hydroxylamino-4,6-dinitrotoluene (2 HA46DNT), 4-hydroxylamino-2,6-dinitrotoluene (4HA26DNT), 2,4-dihydroxylamino-6-nitrotoluene (24DHANT), and the Bamberger rearrangement product of 2,4-dihydroxylamino-6-nitrotoluene. In the presence of saturating CO, CODH catalyzed the conversion of TNT to two monohydroxylamino derivatives (2HA46DNT and 4HA26DNT), with 4HA26DNT as the dominant isomer. These derivatives were then converted to 24DHANT, which slowly converted to the Bamberger rearrangement product. Apparent K{sub m} and k{sub cat} values of TNT reduction were 165 {+-} 43 {micro}M for TNT and 400 {+-} 94 s{sup {minus}1}, respectively. Cyanide, an inhibitor for the CO/CO{sub 2} oxidation/reduction activity of CODH, inhibited the TNT degradation activity of CODH.

• Research Organization: Rice Univ., Houston, TX (US)
• OSTI ID: 20026718
• Journal Information: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 4 Vol. 66; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE
54 ENVIRONMENTAL SCIENCES
BIODEGRADATION
CARBON MONOXIDE
CLOSTRIDIUM
ENZYME ACTIVITY
OXIDOREDUCTASES
REMEDIAL ACTION
TNT