Characterization of lanthanide ions binding sites in the cell wall of Pseudomonas aeruginosa

Journal Article · Tue Feb 15 04:00:00 EST 2000 · Environmental Science and Technology

DOI:https://doi.org/10.1021/es990668h· OSTI ID:20020732

Texier, A C; Andres, Y; Illemassene, M; Le Cloirec, P

Earlier studies have shown that Pseudomonas aeruginosa can adsorb selectively La{sup 3+}, Eu{sup 3+}, and Yb{sup 3+} from aqueous solution. These bacterial cells may find promising applications for removal and separation of lanthanide ions from contaminated effluents. In this work, potentiometric titrations and time-resolved laser-induced fluorescence spectroscopy were used to determine the binding sites of the biomass and, consequently, to elucidate the underlying mechanisms involved in the biosorption of lanthanide ions. Around 90 {+-} 5% of the adsorbed lanthanum was easily desorbed with an EDTA 0.1 M solution. In most instances, lanthanides seemed to concentrate extracellularly. The diversity of potential metal-binding groups was revealed by potentiometric titrations of the biomass. The amount of strong and weaker acidic functional groups in the wet biomass was estimated at 0.24 {+-} 0.04 and 0.86 {+-} 0.02 mequiv/g, respectively. Time-resolved laser-induced fluorescence spectroscopy on europium-loaded P. aeruginosa biomass suggests that europium binding occurs mostly through carboxyl and phosphate groups.

• Research Organization: Ecole des Mines de Nantes (FR)
• OSTI ID: 20020732
• Journal Information: Environmental Science and Technology, Journal Name: Environmental Science and Technology Journal Issue: 4 Vol. 34; ISSN ESTHAG; ISSN 0013-936X
• Country of Publication: United States
• Language: English

Related Subjects

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION
ADSORPTION
AQUEOUS SOLUTIONS
CELL WALL
CHEMICAL COMPOSITION
EUROPIUM
INDUSTRIAL WASTES
LANTHANUM
LIGANDS
MATERIALS RECOVERY
PSEUDOMONAS
RECEPTORS
WASTE PROCESSING
YTTERBIUM