Structure of conjugated bile salt-fatty acid-monoglyceride mixed colloids: Studies by small-angle neutron scattering

Journal Article · Thu Jan 20 04:00:00 EST 2000 · Journal of Physical Chemistry B: Materials, Surfaces, Interfaces, amp Biophysical

DOI:https://doi.org/10.1021/jp992157n· OSTI ID:20017482

Hjelm, R P; Schteingart, C D; Hofmann, A F; Thiyagarajan, P

The structures of particles found in isotropic phases of mixed surfactant systems consisting of conjugated bile salts and fatty lipids were assessed using small-angle neutron scattering. The conjugated bile salts were either cholylglycine or chenodeoxycholylglycine. The fatty lipids were mixtures of oleate and oleic acid either alone or with monoolein. The scattering data suggested that both particle interactions and polydispersity must be modeled in these systems. Particle interactions were modeled using the reduced mean spherical approximation and the decoupling approximation. Maximum entropy was used to characterize the polydispersity. A self-consistent analysis of the scattering was arrived at by making an initial estimate of particle size and shape using derivative-log and Guinier analysis and refining the estimates by analyzing the particle interactions and polydispersity and iterating. There are sufficient similarities in the particle morphologies of these and other conjugated bile salt-fatty lipid systems to suggest a common mode of self-assembly. These solutions are models for bile in the bilary system and intestine content during triglyceride digestion; the common themes of self-assembly have implications for the physiology of lipid solubilization in bile as well as intestinal absorption of dietary lipids.

• Research Organization: Los Alamos National Lab., NM (US)
• Sponsoring Organization: US Department of Energy; National Institutes of Health
• DOE Contract Number: W-7405-ENG-36; W-31109-ENG-38
• OSTI ID: 20017482
• Journal Information: Journal of Physical Chemistry B: Materials, Surfaces, Interfaces, amp Biophysical, Journal Name: Journal of Physical Chemistry B: Materials, Surfaces, Interfaces, amp Biophysical Journal Issue: 2 Vol. 104; ISSN 1089-5647; ISSN JPCBFK
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BILE
COLLOIDS
INTESTINAL ABSORPTION
OLEIC ACID
PARTICLE INTERACTIONS