Accumulation of Antifreeze Proteins on Ice Is Determined by Adsorption

Thosar, Aniket U.; Shalom, Yitzhar; Braslavsky, Ido; Drori, Ran; Patel, Amish J.

doi:10.1021/jacs.3c02705

Accumulation of Antifreeze Proteins on Ice Is Determined by Adsorption

Journal Article · Tue Aug 01 00:00:00 EDT 2023 · Journal of the American Chemical Society

DOI:https://doi.org/10.1021/jacs.3c02705· OSTI ID:2001519

Thosar, Aniket U. ^[1]; Shalom, Yitzhar ^[2]; ^[3]; ^[2]; ^[4]

University of Pennsylvania, Philadelphia, PA (United States); University of Pennsylvania
Yeshiva University, New York, NY (United States)
Hebrew University of Jerusalem, Rehovot (Israel)
University of Pennsylvania, Philadelphia, PA (United States)

Antifreeze proteins (AFPs) enable the survival of organisms in freezing environments by binding to ice crystals and suppressing their growth. The rate at which AFPs accumulate on ice is determined by an interplay between their diffusion from the aqueous solution and their adsorption to the ice crystal. To interrogate the relative importance of these two processes, we combined fluorescence experiments with reaction–diffusion modeling. We found that as AFPs accumulate on ice, their concentration in the aqueous solution does not develop a gradient but remains equal to its bulk concentration throughout our experiments. Furthermore, these findings led us to conclude that AFP accumulation on ice crystals, which are smaller than 100 μm in radius, is not limited by the diffusion of AFPs, but by the kinetics of AFP adsorption.

View Accepted Manuscript (DOE)

Research Organization:: University of Pennsylvania, Philadelphia, PA (United States)

Sponsoring Organization:: Donors of the American Chemical Society Petroleum Research Fund; National Science Foundation (NSF); USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0021241

OSTI ID:: 2001519

Journal Information:: Journal of the American Chemical Society, Journal Name: Journal of the American Chemical Society Journal Issue: 32 Vol. 145; ISSN 0002-7863

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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