Irreversible binding of biologically reduced 2,4,6-trinitrotoluene to soil
Journal Article
·
· Environmental Toxicology and Chemistry
To analyze the fate of 2,4,6-trinitrotoluene (TNT) and its reduction products, a TNT-contaminated soil was spiked with [ring-UL-{sup 14}C]TNT and treated in a laboratory slurry reactor. During an anaerobic/aerobic treatment, the total radioactivity measured in the supernatant and methanolic soil extracts decreased to 2%. The decrease corresponded to an increase of strongly bound radioactivity to the soil. Throughout the whole treatment process, mineralization of TNT was not observed. During the reductive process, unidentified polar substances increased to a maximum amount of 23.2% of the total radioactivity on the day after the start of the experiment. After the end of the anaerobic phase, still 9.7% of the radioactivity was found in this fraction. Only during the aerobic phase did the polar substances disappear completely. The irreversible character of the binding of the reduced metabolites of TNT to the soil was indicated by the failure of desorption even under rigorous and long-term extraction conditions. A significant release of radioactivity could be measured only by using high concentrations of HCl or EDTA. However, in none of the extracts were TNT or any reduced metabolites detected by HPLC and {sup 14}C-radiocounting. Size-exclusion chromatography of humic acids extracted from the treated soil indicated that the metabolites of TNT were evenly bound to the complete range of molecular size of the humic acids.
- Research Organization:
- Fraunhofer-Inst. fuer Grenzflaechen- und Bioverfahrenstechnik, Stuttgart (DE)
- OSTI ID:
- 20012745
- Journal Information:
- Environmental Toxicology and Chemistry, Journal Name: Environmental Toxicology and Chemistry Journal Issue: 11 Vol. 18; ISSN ETOCDK; ISSN 0730-7268
- Country of Publication:
- United States
- Language:
- English
Similar Records
Covalent binding of reduced metabolites of [{sup 15}N{sub 3}]TNT to soil organic matter during a bioremediation process analyzed by {sup 15}N NMR spectroscopy
Hydrolytic release of bound residues from composted soil contaminated with 2,4,6-trinitrotoluene
Two-stage biotransformation of 2,4,6-trinitrotoluene
Journal Article
·
Tue Dec 14 23:00:00 EST 1999
· Environmental Science and Technology
·
OSTI ID:20006576
Hydrolytic release of bound residues from composted soil contaminated with 2,4,6-trinitrotoluene
Journal Article
·
Sun Jun 01 00:00:00 EDT 1997
· Environmental Toxicology and Chemistry
·
OSTI ID:514595
Two-stage biotransformation of 2,4,6-trinitrotoluene
Conference
·
Sat Dec 30 23:00:00 EST 1995
·
OSTI ID:490982