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Respiration-driven methanotrophic growth of diverse marine methanogens

Journal Article · · Proceedings of the National Academy of Sciences of the United States of America
 [1];  [2];  [2];  [2];  [2];  [2];  [2];  [3]
  1. Shandong Univ., Qingdao, Shandong (China); Shandong Univ., Suzhou, Jiangsu (China)
  2. Shandong Univ., Qingdao, Shandong (China)
  3. Pennsylvania State Univ., University Park, PA (United States)
+ Show Author Affiliations
Anaerobic marine environments are the third largest producer of the greenhouse gas methane. The release to the atmosphere is prevented by anaerobic ‘methanotrophic archaea (ANME) dependent on a symbiotic association with sulfate-reducing bacteria or direct reduction of metal oxides. Metagenomic analyses of ANME are consistent with a reverse methanogenesis pathway, although no wild-type isolates have been available for validation and biochemical investigation. Herein is reported the characterization of methanotrophic growth for the diverse marine methanogens Methanosarcina acetivorans C2A and Methanococcoides orientis sp. nov. Growth was dependent on reduction of either ferrihydrite or humic acids revealing a respiratory mode of energy conservation. Acetate and/or formate were end products. In conclusion, reversal of the well-characterized methanogenic pathways is remarkably like the consensus pathways for uncultured ANME based on extensive metagenomic analyses.
Research Organization:
Pennsylvania State Univ., University Park, PA (United States)
Sponsoring Organization:
National Natural Science Foundation of China; Natural Science Foundation of Jiangsu Province; Natural Science Foundation of Shandong Province; USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)
Grant/Contract Number:
FG02-95ER20198
OSTI ID:
2001164
Alternate ID(s):
OSTI ID: 2419448
Journal Information:
Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 39 Vol. 120; ISSN 1091-6490; ISSN 0027-8424
Publisher:
National Academy of SciencesCopyright Statement
Country of Publication:
United States
Language:
English

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59 BASIC BIOLOGICAL SCIENCES
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archaea
biological sciences
global warming
methane
microbiology
reverse methanogenesis
science & technology