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Title: The relationship between ethylene binding and dominant insensitivity conferred by mutant forms of the ETR1 ethylene receptor

Abstract

Ethylene responses in Arabidopsis are mediated by a small family of receptors, including the ETR1 gene product. Specific mutations in the N-terminal ethylene-binding domain of any family member lead to dominant ethylene insensitivity. To investigate the mechanism of ethylene insensitivity, the authors examined the effects of mutations on the ethylene-binding activity of the ETR1 protein expressed in yeast. The etr1-1 and etr1-4 mutations completely eliminated ethylene binding, while the etr1-3 mutation severely reduced binding. Additional site-directed mutations that disrupted ethylene binding in yeast also conferred dominant ethylene insensitivity when the mutated genes were transferred into wild-type Arabidopsis plants. By contrast, the etr1-2 mutation did not disrupt ethylene binding in yeast. These results indicate that dominant ethylene insensitivity may be conferred by mutations that disrupt ethylene binding or that uncouple ethylene binding from signal output by the receptor. Increased dosage of wild-type alleles in triploid lines led to the partial recovery of ethylene sensitivity, indicating that dominant ethylene insensitivity may involve either interactions between wild-type and mutant receptors or competition between mutant and wild-type receptors for downstream effectors.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (US)
Sponsoring Org.:
National Science Foundation (NSF); USDOE
OSTI Identifier:
20006208
DOE Contract Number:  
FG02-91ER20029
Resource Type:
Journal Article
Journal Name:
Plant Physiology (Bethesda)
Additional Journal Information:
Journal Volume: 121; Journal Issue: 1; Other Information: PBD: Sep 1999; Journal ID: ISSN 0032-0889
Country of Publication:
United States
Language:
English
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; ETHYLENE; RECEPTORS; ARABIDOPSIS; SENSITIVITY; BIOCHEMICAL REACTION KINETICS; GENE REGULATION; GENE MUTATIONS

Citation Formats

Hall, A.E., Chen, Q.G., Findell, J.L., Schaller, G.E., and Bleecker, A.B. The relationship between ethylene binding and dominant insensitivity conferred by mutant forms of the ETR1 ethylene receptor. United States: N. p., 1999. Web. doi:10.1104/pp.121.1.291.
Hall, A.E., Chen, Q.G., Findell, J.L., Schaller, G.E., & Bleecker, A.B. The relationship between ethylene binding and dominant insensitivity conferred by mutant forms of the ETR1 ethylene receptor. United States. doi:10.1104/pp.121.1.291.
Hall, A.E., Chen, Q.G., Findell, J.L., Schaller, G.E., and Bleecker, A.B. Wed . "The relationship between ethylene binding and dominant insensitivity conferred by mutant forms of the ETR1 ethylene receptor". United States. doi:10.1104/pp.121.1.291.
@article{osti_20006208,
title = {The relationship between ethylene binding and dominant insensitivity conferred by mutant forms of the ETR1 ethylene receptor},
author = {Hall, A.E. and Chen, Q.G. and Findell, J.L. and Schaller, G.E. and Bleecker, A.B.},
abstractNote = {Ethylene responses in Arabidopsis are mediated by a small family of receptors, including the ETR1 gene product. Specific mutations in the N-terminal ethylene-binding domain of any family member lead to dominant ethylene insensitivity. To investigate the mechanism of ethylene insensitivity, the authors examined the effects of mutations on the ethylene-binding activity of the ETR1 protein expressed in yeast. The etr1-1 and etr1-4 mutations completely eliminated ethylene binding, while the etr1-3 mutation severely reduced binding. Additional site-directed mutations that disrupted ethylene binding in yeast also conferred dominant ethylene insensitivity when the mutated genes were transferred into wild-type Arabidopsis plants. By contrast, the etr1-2 mutation did not disrupt ethylene binding in yeast. These results indicate that dominant ethylene insensitivity may be conferred by mutations that disrupt ethylene binding or that uncouple ethylene binding from signal output by the receptor. Increased dosage of wild-type alleles in triploid lines led to the partial recovery of ethylene sensitivity, indicating that dominant ethylene insensitivity may involve either interactions between wild-type and mutant receptors or competition between mutant and wild-type receptors for downstream effectors.},
doi = {10.1104/pp.121.1.291},
journal = {Plant Physiology (Bethesda)},
issn = {0032-0889},
number = 1,
volume = 121,
place = {United States},
year = {1999},
month = {9}
}